Manipal University 2009 M.Pharm Part-I - exam paper
Manipal University
M.Pharm Part-I Degree Examination- May/June 2009
Subject: Modern Pharmaceutical Biotechnology(PBT 603)
Specialisation: Pharmaceutical Biotechnology
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MANIPAL UNIVERSITY M. PHARM. PART-I DEGREE EXAMINATION - MAY/JUNE 2009 SUBJECT: MODERN PHARMACEUTICAL BIOTECHNOLOGY (PBT 603) SPECIALIZATION: PHARMACEUTICAL BIOTECHNOLOGY
Saturday, May 30, 2009 : 10:00-13:00 Hrs. Max. Marks: 100
Time
IA.
IB.
2A.
2B.
3A.
3B.
4A.
4B.
5A.
5B.
' 5C.
Answer all the questions.
Define MDRTB. Discuss the strategies for TB,vaccine development and challenges involved with a note of few promising candidates in clinical trail.
Explain the role of Dendritic cells and Cytokines in designing vaccines. Add a note on enhancement of co-stimulation in therapeutic vaccine design and Reverse vaccinology.
(10+10 = 20 marks)
Explain the limitations of bacteria as host in recombinant DNA technology and suggest the alternative and better hosts.
With regard to the production of hepatitis B vaccine, explain the following with reasoning:
i) selection of host
ii) selection of vector
iii) Fermentation conditions.
(10+10 = 20 marks)
What are the advantages of immobilization of enzymes? Discuss the entrapment method in detail.
Explain the chemical treatment methods for extracting an enzyme. Outline the purification method of an extracted enzyme.
(10+10 = 20 marks)
Explain Sanger and Maxam Gilbert methods for DNA sequencing.
Explain the mechanism, advantages and disadvantages of RNAi. Add a note on polyethylenimine modifications and R1SC-RNA induced silencing complex.
(10+10 = 20 marks)
Discuss the role of Pharmacogenetics and Pharmacogenomics in drug metabolism.
Give an account of Patterns of Stem cell differentiation and assessment of Human stem cell safety in Stem Cell Research.
Schematically represent direct and indirect ELISA.
(10+5+5 = 20 marks)
PBT 603 Page 1 of 1
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