Janardan Rai Nagar Rajasthan Vidyapeeth 2006 B.Tech Biotechnology INDUSTRIAL MICRO_BIOLOGY-II - Question Paper

BTBT1- INDUSTRIAL MICRO_BIOLOGY-II

Time : 3 hrs Maximum marks : 75

 

Instruction:

1. Question paper is divided into Group A, Group B and Group C

2. Each Group is 25 Marks

3. Figure to the right in bracket indicates marks.

4. Good Handwriting is expected

5. Assume suitable data if necessary.

 

Group A (25 Marks)

 

Answer any three questions (Question 1 is compulsory)

	(5)

 

(10)

Q.1 Write a uses of microbial Biomass in foods and Feed ?

Q.2 Describe about substrate and Nutrient Requirements in Microbial Biomass ?

(10)

Q.3 Write a detailed notes on :-

1. Enzyme Immobilization

2. Cellulose

(10)

Q.4 Define and explain

1. Enzyme Activity 2. Enzyme Inhibitors 3. Enzyme Assay

(10)

Q.5 How Enzymes are produce on large scale ?

 

Group B (25 Marks)

 

Answer any three questions (Questions 6 is compulsory)

	(5)

 

Q.6 Explain Genetic improvement in Citric Acid- producing microorganism ?

(10)

Q.7 How Bacteria produce Citric Acid and explain the recovery methods ?

	(10)

 

Q.8 Explain how Glutamic Acid is produced from Carbohydrate ?

	(10)

 

Q.9 What is submerged Vinegar fermentation . Explain in detail ?

	(10)

 

Q.10. Explain atleast 3 raw materials for Ethanol Fermentation ?

 

Group C (25 Marks)

 

All Questions are Compulsory.

 

Q.11 Fill in the blanks ( each question carry 2 marks)

1. Detection of ______ increased productivity is difficult .

2. Bakers yeast requires ________ for growth , and compressed yeast.

3. ________ processing includes the recovery of biomass from the fermentor.

4. Citric acid production from ________ is similar to that from n-paraffins.

5. The pH value optimal growth and Glutamic acid production is ___________

 

Q.12 Multiple choice question. (Each question carry 2 marks)

1. The optimal value of kd for glutamic acid fermentation is _______-

a) 3.5 x 10^-6 (mol O₂ ) atm^-1 min^-1 ml^-1 b) 3.5 x 10⁶ (mol O₂) atm^-1 min^-1 ml^-1

c) 7.8 x 10^-6 (molO₂) atm^-1 min^-1 ml^-1 d) 7.8 x 10⁶ (molO₂) atm^-1 min^-1 ml^-1

 

2. _________ convert ethanol to L- glutamic acid with yield of 60%.

a) Brevibacterium paraffineus b) Brevibacterium Flavum c) Brevibacterium sp. B136

d) Brevibacterium erythropolis.

 

3. Optimal carbon : Nitrogen ratios for valine production seems to be ________

a) Approx 50 :7 to 50 :4 b) Approx 100 :7 to 100 : 4 c) Approx 1000 :7 to 1000: 4

d) Approx 25 : 7 to 25 : 4

 

4. _________ is the undesirable oxidation of acetic acid to carbon dioxide and water .

a) Concentration b) pH c) Temperature d) Over oxidation

 

5. Foams which are formed in fermentor , must be prevented is called ________

a) Reformer b) Aerator c) Deformer d) Acetator

 

Q.13 True or false (each question carry 1 marks).

1. Enzyme substilisin and carrier matrix is cellulose is an examples of enzyme immobilized by

adsorption .

2. A niger needs a variety of divalent trace elements for growth.

3. Magnesium is not essential for a varity of enzyme reaction in the cell.

4. Most of the wild type organisms capable of copious valine production belong to the family

staphylococcus.

5. In Pseudomonas sp. No. 483 alanine is formed from pyruvate by the action of alanine

dehydrogenase.

 

 

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