Janardan Rai Nagar Rajasthan Vidyapeeth 2006 B.Tech Biotechnology BIOINFORMATIC - Question Paper

Model Question Paper

BTBT3- BIOINFORMATIC

Time : 3 hrs Maximum marks : 75

 

Instruction:

1. Question paper is divided into Group A, Group B and Group C

2. Each Group is 25 Marks

3. Figure to the right in bracket indicates marks.

4. Good Handwriting is expected

5. Assume suitable data if necessary.

 

Group A (25 Marks)

 

Answer any three questions (Question 1 is compulsory)

	(5)

 

Q.1 Define Bioinformatics and Give its objectives and applications ?

(10)

Q.2 Explain in detail the structure of DNA along with Double Helix structure ?

(10)

Q.3 Explain DNA replication is semi-conservation ?

(10)

Q.4 What are the consideration and Application of multiple alignment ?

(10)

Q.5 Give the methods of structure predication of proteins for known folds ?

 

Group B (25 Marks)

 

Answer any three questions (Questions 6 is compulsory)

	(5)

 

Q.6 Define Proteomics and write down proteome analysis approaches ?

(10)

Q.7 Write an overview of Bioinformatics applications ?

	(10)

 

Q.8 Briefly Explain BLAST ?

	(10)

 

Q.9 Explain the methods of calculation of distance matrices ?

	(10)

 

Q.10.Write Notes on :-

1. DNA cloning 2. Maxam Gilbert chemical Sequencing

 

 

Group C (25 Marks)

 

All Questions are Compulsory.

 

Q.11 Fill in the blanks ( each question carry 2 marks)

 

1. ________ is a system for finding ungapped local multiple alignment in protein sequence.

2. A _________ is a 2 Dimensional graph showing evolutionary relationship among organism.

3. The principle of the _______ is to build up a multiple alignment by constructing a tree and

aligning sequence and pre-aligned groups of sequence simultaneously.

4. _________ compares 3 D co-ordinates of a newly determined protein structure to those in the

MMDB/ PDB database.

5. _______ is based on statistical analysis of inheritance patterns and gives a relative ordering of

markers used.

 

Q.11 Multiple choice question. (Each question carry 2 marks)

 

1. The primary sequence of a protein is held together by _________

a) Ionic bond b) hydrogen bond c) Hydrophobic Interaction d) Covalent peptide bonds.

2. When a corresponding character is shared between two species or population , that character is

said to be __________

a) Identical b) Homogeous c) Similar d) Heterogenous

3. The degree to which two species or populations shares identities is called _____

a) Similar b) Identical c) Orthologous d) Analogous

4.__________ is when characters are homologous with divergent function.

a) Analogous b) Paralogous c) Homologous d) Orthologous

5. The notion of _________ derives its definition from the concept of mutation , by assigning

weights to each mutation.

a) Space b) Insertion c) Distance d) Sequence.

 

Q.11 True or false (each question carry 1 marks).

 

1. Mutations of amino acid does not survive if they do not change the 3 D structure of the folded

protein.

2. RasMol is one of the popular tools for protein structure visualization , it reads molecular structure

files from the Protein Data Bank (PDB).

3. If the database search with U picked a homologue that has known 3D structure one can predict

3 D structure for U by homology modeling .

4. Hidden Markov models are used for DNA distance matrices and are calculated such that each

mismatch between two sequences.

5. In the B-form of DNA, major Groove is on the opposite side from the minor groove.

 

 

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