Madhya Pradesh Bhoj Open University 2007 B.Sc Biochemistry bio chemistry - Question Paper

NOVEMBER 2007 14454/NRD
Time : 3 hours Maximum : 100 Marks

part A – (10 X three = 30 marks)

ans any TEN ques.

All ques. carry equal marks

every ans should not exceed 50 words

1. What is a reference electrode? provide 2 examples.
2. Comment on redox dyes.
3. Explain osmosis and mention its uses.
4. What happens on adding a drop of blood to a hypotonic solution?
5. Differentiate rate zonal and isopyenic centrifugation.
6. List out the various kinds of adsorbents used in adsorption chromatography and provide their uses.
7. Give the principle of immunoelectrophoresis and mention its applications.
8. How does a spectrophotometer differ from a colorimeter.
9. Explain stable and radioactive isotopes with example.
10. Write about the primary and secondary fluors used in Liquid Scintillation counters.
part B – (5 X six = 30 marks)

ans any 5 ques.

All ques. carry equal marks

every ans should not exceed 300 words

11. Explain a glass electrode and its function in pH meter.
12. Describe tissue slice technique.
13. Enumerate the kinds of rotors used in ultracentrifuges and their uses.
14. Describe the principle and application of gas liquid chromatography.
15. Give an account of Tiseluis moving boundary electrophoresis.
16. Explain how flame photometer is used in the determination of trace elements.
17. Write about GM counter and its applications.


part C – (2 X 20 = 40 marks)

ans BOTH ques.

All ques. carry equal marks

every ans should not exceed 1200 words

18. (a) Write about differential centrifugation and its applications.
Or
(b) discuss the principle and application of paper electrophoresis.

19. (a) provide an account of the principle and instrumentation of spectrofluorimetry. How is the method useful in determining the content of Riboflavin and Thiamine?
Or
(b) explain different biochemical applications of radioisotopes.

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Earning:   Approval pending.