Andhra University 2007 M.Sc BioTechnology MICROBIOLOGY - Question Paper

Andhra university

[SMB-S-304]

M.Sc. DEGREE exam

Third Semester

Microbiology

MOLECULAR BIOTECHNOLOGY
(Revised regulatons effective from 2005-2006 admitted batch)

Time: 3 hours Maximum:85 marks

1. Write short notes on any 5 of the following:
(5*5=25)

(a) Southern blot analysis
(b) Thermostable DNA polymerases
(c) DNA ligases
(d) Adaptor oligonucleotides
(e) Bacterial expression vector
(f) In vivo gene therapy
(g) DNA microarrays
(h) Applications of DNA chips

UNIT-I

2.
(a) Distinguish ranging from type-I, II,III restriction endonucleases. Add a note on their applications. . (15)

Or
(b) Write various approaches for isolation of intact genomic DNA


UNIT-II

3.
(a) Briefly explain the strategies for cloning of foreign genes in E.coli system

Or (15)

(b) How do you identify the recombinant clones expressing proteins

UNIT-III

4.
(a) discuss the expression of cloned foreign genes in animal cell system (15)

Or

(b) explain the social and ethical implications of recombinant DNA technology in biological sciences.

UNIT-IV

5.
(a) How do you prepare nucleic acid probes with various labels? Add a note on their applications (15)

Or

(b) Write on preparation of protein microarrays. elaborate the advantages and disadvantages of DNA and protein microarrays

Earning:   Approval pending.