Andhra University 2007 B.Sc Chemistry BIO-TECNOLOGY - Question Paper

[BS – 111]

PART II – BIO-TECNOLOGY
(Common Core Syllabus)

Time : 3 hours
Maximum : 100 marks

part A
(10 x 2.5 = 25 marks)

ans any TEN of the subsequent.

1. (a) pH
(b) Sexduction.
(c) Bacterial transformations.
(d) Plasmids.
(e) Lampbrush chromosome.
(f) Functions of DNA.
(g) Crossing over.
(h) Nullisomy.
(i) Rocket-electrophoresis.
(j) Autoradiography.
(k) Transduction.
(l) Single cell protein.

part B
(6 X five = 30 marks)

ans and 6 of the subsequent.

1. (a) Bioreactors
(b) Flourimetry.
(c) Paper chromatograph.
(d) Chromosome mapping.
(e) Conjugation in Bacteria.
(f) Transposable elements.
(g) Isoelectric focussing.
(h) Affinity material.

part C
(3 x 15 = 45 marks)

ans any 3 ques..

2. discuss in detail the immobilization of enzymes with suitable examples and its application.

3. Write an account of gene transfer mechanism in procaryotic cells.

4. define the method of separation of proteins using SDS – polyacrylamid gel electrophoresis and the principles involved there in.

5. discuss the scope and multidisciplinary nature of ‘Biotechnology’.

6. define the basic principles and applications of High Pressure Liquid Chromatography (HPLC)

Earning:   Approval pending.