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Veer Narmad South Gujarat University 2010 M.Sc BioTechnology IBT-401 : Biochemistry -3 (sem.- 4) (Regular) . - Question Paper

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RM-3807

M. Sc. (Sem. IV) (Biotechnology) (Regular) Examination May/June - 2010 IBT 401 : Biochemistry - III

(New Course)

Time : 3 Hours]

[Total Marks : 70

"X Seat No.


Instructions :

(1)

r M CnsLLnlcj.L{l [qoicu u? snqw <h>h41.

Fillup strictly the details of signs on your answer book.

Name of the Examination :

M. Sc. (Sem. 4) (Biotechnology) (Regular)

Name of the Subject:

IBT 401 : Biochemistry - 3

-Subject Code No.:

3

OO

0

7

-Section No. (1,2,.....): 1&2

(2)    Figures to the right indicate full marks of the question.

Student's Signature


(3)    Draw neat and labeled diagrams whenever necessary.

(4)    Both sections must be written in separate answer books.

SECTION I

Q.l Answer the following: -    (05)

1.    Define cofactors.

2.    Which enzyme has highest turnover number?

3.    Mention some activated carriers in metabolism.

4.    Write significance of NAD+ and NADP+.

5.    Define constitutive enzymes.

Q.2 Give structures of ten metabolite precursors.    (10)

Q.2


(06)

(04)

(10)


Q.3

Q.3

(a)

(b) Q.4

(a)

(b)

(c)

(d)


(04)

(05) (10)


OR

Describe the following: -

(a).    Explain role of ATP in metabolism.

(b).    IUB classification of enzymes.

Describe lock & key model of enzyme action. Compare it with induced fit mechanism.

OR

Describe the following: -

Effect of temperature and pH on enzyme activity.

Isotopic labeling to study biochemical pathways.

Write brief Notes on Following: - (Any TWO) (05 x 02 = 10) Allosteric enzymes.

Graphical representation of M.M equation.

Integration of metabolic pathways at cellular level.

Salient features of enzymes.


Q.l

Answer the following Questions: -

1

Define formimino group.

2

What is GABA?

3

What is the payoff phase?

4

What is carnitine shuttle?

5

Define fermentation.

Q-2

Describe: -

(a)

Regulation of glycolysis at PFK-1.

(b)

TCA cycle and its regulation.

OR

Q-2

Describe: -

(a)

Significance of pentose phosphate pathway.

(b)

Synthesis of Palmitic acid.

Q-3

Describe in detail gluconeogenesis.

OR

(05)

(05)

(04)

(06)

(10)

(10)

(10)

(05) (05)


Q.3 Explain at length beta oxidation of fatty acid.

Q.4 Explain in detail synthesis of glutamate family.

OR

Q.4 Describe:-

(a).    Biosynthesis of Histidine.

(b).    Significance of NADPH.

RM-3807]    2    [ 400 ]







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