Kannur University 2011 B.Sc MicrobiologyB.S.C biotechnology (2c02BTC) - Question Paper

part A(1*2=2 WTAGE)

1.A.WHAT IS THE TEMPERATURE OPTIMA OF TAQ DNA POLYMERASE
B.TEMPERATURE RANGE AT WHICH COHESIVE END LIGATION IS PERFORMED
C.WHICH IS TRANSACTING ELEMENT NEEDED FOR TDNA TRANSFER
D.WHICH IS NOT TELOGEN(ddTTP,ddATP,ddCTP,ddGTP)
2.A.COFACTOR FOR T4 DNA LIGASE
B.A TEMPLATE INDEPENDENT POLYMERASE
C.WHICH AMONG THE subsequent IS tDNA VECTOR
D. WHICH AMONG THE subsequent IS A MARKER GENE USED IN TRANSGENIC PLANTS
( tetR,ampR, cmR,hotII)

part B (ANY nine )(1*9=9WTAGE)
1.BIOLISTICS
2.TRANSFER DNA
3.VNTR
4.RESTRICTION ENDONUCLEASE
5.PLAQUE LIFT
6.DISARMING
7.AGROBACTERIUM RHIZOGENES
8.LIPOSOMES
9.SYNTENY
10.S35 LABELLING

part C (ANY 4)(2*4=8)

1.BINARY VECTORS
2.AUTOMATED DNA SEQUENCING
3.CLONING OF MITOCHONDRIAL GENES
4.COCULTURING
5.WESTERN BLOTTING
6.MAXAM AND GILBERT SEQUENCING

part D (ANY 2)(2*4=8)
1.CDNA STEPS
2.GENE TRANSFER METHOD FOR TRANSGENIC PLANTS
3.DIFFERENT TEMPERATURE REGIMES IN PCR CYCLES ? WHAT IS THE SIGNIFICANCE OF every
4.APPLICATIONS OF DNA FINGERPRINTING

Earning:   Approval pending.