Kakatiya University (KU) 2011-3rd Year B.Sc Biotechnology Practical Bank - Question Paper

Kakatiya University
Practical ques. Bank
B.Sc. Biotechnology
III year

Paper – III

Time: three hrs Max.Marks:50

Q.I. a) Isolation of DNA from plant cells eight Marks
b) Isolation of DNA from animal cells
c) Isolation of DNA from bacterial cells

Q.II. a) Analysis of DNA by agarose get electrophoresis eight Marks
b) Restriction digestion of DNA

Q.III. a) Preparation of competent cells of bacteria eight Marks
b) Bacterial Transformation
c) Selection of Transformants under pressure (antibiotics)

Q.IV. a) Immuno diffusion Test eight Marks
b) ELISA Test
c) Microagglutination using microtiter plates (ABO & Rh blood groups)
d) Viability Tests of cells/bacteria (Evans blue test or Trypan blue test)
e) Coomb's Test

Q.V. Spotting (4 x two ½) 10 Marks

Q.VI. Record four Marks

Q.VII. Viva-voce four Marks



B.Sc. Biotechnology-III Year
Practical Paper-III

Q.V. Spotting:
1. Cot curve
2. Mitochondrial genome (Human)
3. Chloroplast genome (Tobacco)
4. Eukaryotic Promoter
5. VNTRs
6. RNA polymerase
7. RNA – splicing
8. t-RNA
9. Pre-initiation complex
10. Lac-Operon
11. Restriction Enzymes
12. PBR 322 & PUC
13. Genomic and C-DNA libraries
14. Blotting techniques (S/N/W)
15. PCR
16. Antigen
17. Immunolglobin
18. ELISA
19. Phagocytosis
20. Lymphosites

Earning:   Approval pending.