Integral University 2009 M.Tech Biotechnology Bioinformaics Genomics and Proteomics - Question Paper
No of Printed Pages - 2 mBJJm
-3
* IIIIiiii
Roll No.
0 111111
THIRD SEMESTER EXAMINATION, 2008-09
BIOINFORMATICS, GENOMICS & PROTEOMICS Tim 3 Hours Total Marks 100
1
Note: (j) Attempt any FIVE questions
(II) Marks are Indicated agalnBt each question.
1- Apply the Needleman - Wunsch Algorithm to determine the best alignment for the following amino add sequences 20
Seq 1 - TWTGRVE Seq 2-TVATRIE Use the match score = +3, mismatch score = -1 and gap penality = -4 3 What ts the score of the optimal alignment? Back trace the matrix to _ show all the optimal alignments possible.
9 2. (a) Explain In detail the shot gun sequencing methodology available y* for DNA sequencing. 10
1 (b) Briefly describe the principle and design of DNA microarray along
! with its specific applications 10
*1 3. List out the various algorithms available for the prediction of secondary
structure of proteins. Discuss in detail the Chou-fasman and GOR algorithms for the same. What are the efficiencies of these methods? 20
- 4. Explain in detail the various insilico gene prediction strategies employed in the case of eukaryotes, along with their methodologies. 20
MTBT-301
5. (a) Define motif and profile Given a set of multiple afcgned
sequences, how wit! you buKd a profile for the same? 10
(b) Write short notes on the following 10
(I) PROSITE
(II) P*am
6. Dtscuss in detail the various expenmentai and the non-homology methods available for the scud/ of pnoteln-protein Interactions 20
7 Write short notes on any Four of the following: 5 x 4 20
() PSI - BLAST
(b) Human Genome Project
(c) ExPASy Proteomics toots
(d) Structure tosed approach to assignment of gene function
(0) KEGG
No of Printed Pages - 2 mBJJm
-3
* IIIIiiii
Roll No.
0 111111
THIRD SEMESTER EXAMINATION, 2008-09
BIOINFORMATICS, GENOMICS & PROTEOMICS Tim 3 Hours Total Marks 100
1
Note: (j) Attempt any FIVE questions
(II) Marks are Indicated agalnBt each question.
1- Apply the Needleman - Wunsch Algorithm to determine the best alignment for the following amino add sequences 20
Seq 1 - TWTGRVE Seq 2-TVATRIE Use the match score = +3, mismatch score = -1 and gap penality = -4 3 What ts the score of the optimal alignment? Back trace the matrix to _ show all the optimal alignments possible.
9 2. (a) Explain In detail the shot gun sequencing methodology available y* for DNA sequencing. 10
1 (b) Briefly describe the principle and design of DNA microarray along
! with its specific applications 10
*1 3. List out the various algorithms available for the prediction of secondary
structure of proteins. Discuss in detail the Chou-fasman and GOR algorithms for the same. What are the efficiencies of these methods? 20
- 4. Explain in detail the various insilico gene prediction strategies employed in the case of eukaryotes, along with their methodologies. 20
MTBT-301
5. (a) Define motif and profile Given a set of multiple afcgned
sequences, how wit! you buKd a profile for the same? 10
(b) Write short notes on the following 10
(I) PROSITE
(II) P*am
6. Dtscuss in detail the various expenmentai and the non-homology methods available for the stud/ of pnotetn-protein Interactions 20
7 Write short notes on any Four of the following: 5 x 4 20
() PSI - BLAST
(b) Human Genome Project
(c) ExPASy Proteomics toots
(d) Structure tosed approach to assignment of gene function
(0) KEGG
|
Attachment: |
| Earning: Approval pending. |
