Integral University 2011 M.Tech Biotechnology Genetic Engineering - Question Paper
No. of Printed Pages - 2 MTBT-204
Roll No.
SECOND SEMESTER EXAMINATION, 2010-11 ENZYME ENGINEERING
Total Marks: 100
Note : (i) Attempt any Five questions.
(ii) Marks are indicated agains1 each question.
1- (a) Give a detailed account of medical applications of enzymes. 10 (b) Derive Nuchaelis-Menten equation and discuss the factors affecting rate of enzyme catalyzed reactions. * 10
2. (a) Define enzyme kinetics. Derive the rate of a reaction using Briggs-Haldane steady state approach. 10
(b) Draw the Lineweaver Burk Plot for the different types of enzyme inhibition and also compare these inhibitions with respect to their Km and Vmax values. 10
(a) What do you understand by the immobilization of enzymes? Discuss the kinetics of immobilized enzymes. 10
(b) Write a note on : 10 *
(i) Glucose oxidase
(ii) Lysozyme
10
10
(a) Write short notes on enzyme reactors.
(b) Compare the kinetics of different enzyme reactors,
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5., Discuss in brief how immobilization has a direct effect on enzyme
constrain. 10
(b) Write short notes on the enzyme reactions in organic media. 10
6. Write short notes on any Two of the following : 10 x 2 = 20 (a) Intermolecular cross linking
lb) Gel entrapment
=j Parameters of enzyme immobilization
(d) Heterogeneous kinetics
7. Write short notes on any Four of the following: 5 x 4 = 20
(a) Industrial uses of enzymes
Factors effecting enzyme kinetics
(c) Abzymes and their uses
(d) Design of enzyme reactor
(e) Analytical scopes of enzymes *'
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No. of Printed Pages - 2 MTBT-203
Roll No.
f
SECOND SEMESTER EXAMINATION, 2010-11 GENETIC ENGINEERING
Total Marks: 100
Note : (i) Attempt any FIVE questions.
(ii) Marks are indicated against each question.
1. Compare any Four of the following :
5 x 4 = 20
(a) Screenable Vs Auxotrophic markers J&) Type II Vs Type I restriction enzymes
(c) Chemical induction Vs Electroporation
(d) RFLPVsAFLP
(e) Colony hybridization and In-situ hybridization
2- Write in detail about A,-phage and cloning strategy used in it? 10 (b) Discuss the terms linkers and adoplers with suitable examples? 10
/(a) Explain how will you construct c-DNA and genomic library? 10
(b) If you have given a gene of interest of 100kb, what kind of vectors you will choose for cloning and also show the strategy to clone. 10
4. Write short notes on any Four
5 x 4 = 20
(a) Blue-white screening
(b) Western blot
(c) T4-DNAIigase
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((fr Physical methods of transformation (e) RAPD
5. (a) Some scientists are worried that bacteria produced by genetic
engineering might escape from the laboratory into the environment. What problems might arise if this happenes? How can you solve this problem? 10
(b) Suppose it was possible to use genetic engineering to make people more intelligent. Do you think this should be allowed? 10
6. (a) What do you understand by Polymerase Chain Reaction (PCR)?
Describe with a suitable examples showing all the necessary steps and components involved. 10
(b) Write in detail about animal viruses as vector. 10
7. Write short notes on any Four of the following : 5x4 = 20
(a) Stringent plasmids
(b) Agrobacterium mediated transformation
(c) Antisense RNA technology
(d) Polynucleotide kinase
(e) M-13phage
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