Integral University 2010 M.Tech Biotechnology Microbial genetic and engg. - Question Paper
No. of Printed Pages - 2 MTBT-103
Roll No. I I | I | j | | I I
FIRST SEMESTER EXAMINATION, 2010-11 MICROBIOLOGICAL GENETICS AND ENGINEERING
Time : 3 Hours Total Marks : 100
Note : (i) Attempt any FIVE questions.
(ii) Marks are indicated against each question.
1- (a) Describe the nutrional requirement of the four major nutrional
groups and give some major examples of each. 10
(b) Why do microbiologists use chemically defined media? 5
(c) Describe the kinetics for microbial growth in batch culture. 5
2. (a) Discuss the use of the chemostat in obtaining Continuous
cultures. 10
(b) Explain the importance of pH in the growth of micro-organisms. 5
(c) Explain the concept of exponential growth. 5
r
3. Comment on : 5 x 4 = 20
r
(a) Specialized Transduction
(b) Biological Oxygen demand
(c) Synchronous Culture
(d) Complementation
4. Differentiate the following : 5 x 4 = 20
s'
(a) Batch and Continuous s
(b) Thermal death of spores
Chemical and Steam Sterilization
(d) Enriched and Enrichment media
5. Write short notes on: 5 x 4 = 20
(a) Horizontal gene transfer
(b) Replication of RNA tumor viruses
(c) Fed batch culture
(d) Mapping of bacterial genes
6. (a) Determine the kinetic parameters by batch culture. 10
(b) Describe the role of maintenance and endogenous metabolism in substrate utilization and growth. 10
7. (a) Evaluate the kinetics of growth and product formation by filamentous organism. 5
(b) Briefly describe the following: 15
(i) Compartmental and metabolic models.
(ii) Genetic map with an example.
(iii) Factors influencing the antifoam agents.
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| Earning: Approval pending. |
