Integral University 2010 M.Tech Biotechnology Downstream Engineering - Question Paper
No. of Printed Pages - 2 MTBT-202
Roll No.
SECOND SEMESTER EXAMINATION, 2010-11 DOWNSTREAM PROCESSING
>
Time: 3 Hours Total Marks : 100
Note : (i) Attempt any FIVE questions.
(ii) Marks are indicated against each question.
1- (a) Write a note on the purification of proteins by precipitation method. 15
(b) Describe the term "Adsorption and its advantages in down stream processing. Also write about adsorption isotherms. 5
2. Attempt any Five options: 4 x 5 = 20
(a) Salt induced precipitation
(b) Pervaporation
(c) Supercritical fluid extraction
(d) Pulsed - Field gel electrophoresis
(e) Ultrafiltration
(f) Trickling filters
(g) Isoelectric focusing
3. (a) How Downstream processing is essential and important aspect of bioprocess. Support your answer by giving an example. 10 (b) What do you understand by liquid-liquid extraction? Define various criteria on which physical extraction depends. 10
MTBT-202
4. (a) Write a note on the process of crystallization and its use in
downstream processing. 15
(b) Discuss sludge loading for conventional activated sludge
treatment process. 5
i'
i
5. (a) Define various stages of Sewage treatment system. Write about
fluidized expanded bed reator. 10
(b) Write short note on freeze drying and its advantages. 10
6. Affinity chromatography is a method of separating biochemical mixtures
/
based on highly specific biological interaction. Justiiy this statement by explaining principle, working and one application. 20
7. (a) Describe centrifugal sedimentation using mathematical expression
and write about different typo of rotors. 10
(b) Describe different method of cell disruption. 10
2
No. of Printed Pages - 2 MTBT-202
Roll No.
SECOND SEMESTER EXAMINATION, 2010-11 DOWNSTREAM PROCESSING
>
Time: 3 Hours Total Marks : 100
Note : (i) Attempt any FIVE questions.
(ii) Marks are indicated against each question.
1- (a) Write a note on the purification of proteins by precipitation method. 15
(b) Describe the term "Adsorption and its advantages in down stream processing. Also write about adsorption isotherms. 5
2. Attempt any Five options: 4 x 5 = 20
(a) Salt induced precipitation
(b) Pervaporation
(c) Supercritical fluid extraction
(d) Pulsed - Field gel electrophoresis
(e) Ultrafiltration
(f) Trickling filters
(g) Isoelectric focusing
3. (a) How Downstream processing is essential and important aspect of bioprocess. Support your answer by giving an example. 10 (b) What do you understand by liquid-liquid extraction? Define various criteria on which physical extraction depends. 10
MTBT-202
4. (a) Write a note on the process of crystallization and its use in
downstream processing. 15
(b) Discuss sludge loading for conventional activated sludge
treatment process. 5
i'
i
5. (a) Define various stages of Sewage treatment system. Write about
fluidized expanded bed reator. 10
(b) Write short note on freeze drying and its advantages. 10
6. Affinity chromatography is a method of separating biochemical mixtures
/
based on highly specific biological interaction. Justiiy this statement by explaining principle, working and one application. 20
7. (a) Describe centrifugal sedimentation using mathematical expression
and write about different typo of rotors. 10
(b) Describe different method of cell disruption. 10
2
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