Integral University 2011 M.Tech Biotechnology Immunotechnology - exam paper
No. of Printed Pages - 3 MTBT-302(A)
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Roll No. !
THIRD SEMESTER EXAMINATION, 2011-12
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Time : 3 Hours Total Marks : 100
Note : (i) Attempt any FIVE questions.
(ii) Marks are indicated against each question.
(a) Answer the following : 2x5 = 10
(i) The single best chance of a tissue graft being accepted in a
human recipient is if it is :
A. An isograft
B. An allograft given under the cover of potent immunosuppression.
C. A xenograft from a pig in which the gene fro alpha-1, 3-galactosyltransferase has been knocked-out and therefore the Gal epitope eliminated.
D. Given to a recipient that is treated with anti-CD3.
(ii) Each of the following statements concerning a hybridoma
cell is correct EXCEPT :
A. The spleen cell component provides the ability to form antibody.
B. The myeloma cell component provides the ability to grow indefinitely.
C. The antibody produced by a hybridoma cell is IgM, because heavy-chain switching does not occur.
D. The antibody produced by a hybridoma cell is homogeneous; i.e. it is directed against a single epitope.
1 P.T.O.
MTBT-302(A)
(Hi) Which one of the following is the BEST method of reducing the effect of graft-versus-host disease in a bone marrow recipient?
A. Matching the complement components of donor and recipient.
B. Administering alpha interferon
C. Removing mature T cells from the graft
D. Removing pre-B cells from the graft
(iv) Graft and tumor rejection are mediated primarily by :
A. Non-complement-fixing antibodies
B. Phagocytic cells
C. Helper T cells
D. Cytotoxic T cells
(v) A patient with severe asthma suffers due to high level of histamines. The best treatment strategy suggested is :
A. Monoclonal antibodies against IgE
B. Corticosteroidal drugs
C. Recombinant IL-4
D. All of the above.
(b) Give a brief account on : 2x5 = 10
(i) Chimeric toxins (ii) X-linked agammaglobulinemia
(iii) Adjuvants J]v) Xenograft
(v) Serotyping
2. (a) Give a detailed account on mechanism adopted by the HIV virus to infect CD4+T cells. 10
(b) Describe the immune evasion mechanism adopted by the virus to establish itself. 10
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3 Write short notes on any Two of the following : 10 x 2 = 20
(a) DNA vaccines (ii) Stem cell therapy
4. Differentiate between the following : 5 x 4 = 20
(a) TATA and TSTA J Killed and Attenuated vaccines Active and Passive therapy
(d) Chimeric and humanized mtpnoclonal antibodies
5. Describe in detail the basic biology of Type I and Type II interferons and also mention their therapeutic potential. 20
6 Give a detailed protocol of production of mouse monoclonal antibody against lL-2 receptor and also mention its use as a therapeutic agent. 20
OR
Give a detailed account on corticosteroidal and non steroidal drugs and their mechanism of action as anti-inflammatory agents. 20
7 Comment the following : 4 x 5 = 20
( ELISA
(W Hyperacute rejection
(c) IL-20
(d) Antimetabolites
(e) Peptide therapy
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