Integral University 2009 M.Tech Biotechnology Biochemistry and biophysical chemistry - Question Paper
FIRST SEMESTER EXAMINATION, 2009-10 BIOCHEMISTRY AND BIOPHYSICAL TECHNIQUES
Total Marks : 100
Time : 3 Hours
Note : (i) . Attempt any FIVE questions.
(ii) Marks are indicated against each question
1. Attempt any Two of the following :
10 x 2 ~ 20
(a) What is mecint by isotachophoresis? Write in detail how it can be achieved in the SDS-PAGE.
(b) Write a note on Pulse field gel electrophoresis.
(c) What is void volume? Write a brief how ge! permeation is useful in determination of molecular weight of a compound.
2. Attempt any Two of the following : 10x2 = 20
(a) Describe tertiary and quaternary structures of proteins and explain the forces responsible to stabilize these structures.
(b) Give the classification of carbohydrates and write the biological importance of their derivatives.
(c) What are the major features of tRNA structure? What are the functions of each of the arms of a t-RNA molecule?
3. Attempt any Two of the following : 10x2 = 20
(a) What is the importance of Braggs equation in X-ray diffraction
and how X-ray diffraction used in determining the three dimensional structure of a compound.
(b) Write a- brief account on "autoradiography method and discuss ' its applications. . \ C
(cj What is Circular dichroism [CD]? Write the principle and its jiological applications.
*v
Attempt any Two of the following : ' 10-x 2 = 2Ch*
(a) Write the significance of monochromatic light irj spectrophotometry and write a note on the principle of UV-Visr_ spectrophotometry? *
(b) What is the basis for image formation in electron microscopy ancr* write how you made suitable the - specimen for Elec'trort
microscopy.
. .............
(c) What is Infrared spectroscopy? Write how it is useful in the
analysis of the compound. .
s
Attempt any Two of the following : 10x2 = 20
an account of Mass spectrometry in detail laying emphasis
V.* I V
on the principle, instrumentation and uses, .'
(b) Write notes on Spin coupling in NMR. ' C
V
(c) Give an account of biosensors and enumerate on its uses foi>~ diagnostic purposes.
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Attempt any Two of the following : - 10x2 = 20~,
(a) Write a brief account on centrifugal rotors.
(b) Explain, how differentia! centrifugation differs with rate zonal_ centrifugation.
(c) What is sedimentation coefficient and write about the different factors affecting it. , .
JV1TBT- iQI
Attempt any Two of the following : . 10x2 = 20
(a) What is reverse /phase chromatography and how it differs from-normal phase chromatography? Give an elaborative account of
HPLC. *
(b) -What is-flow, cytometry? .Explain how it can be used in cell sorting?
(c) Describe in detail about structure and functions of simple lipids.
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