Integral University 2011 M.Tech Biotechnology Immunotechnology - Question Paper
No. of Printed Pages - 3
Roll No.
MTBT-302(A)
B. Tech. - M. Tech. (DUAL) (BIOTECHNOLOGY)
NINTH SEMESTER EXAMINATION, 2010-11 IMMUNOTECHNOLOGY
Total Marks : 100
Note : (I) Attempt any FIVE questions.
(ii) Marks are indicated against each question.
1. (a) Differentiate between the following :
3x4 = 12
(i) Monoclonal and Polyclonal antibodies (") Redundant and Pleitropic cytokines
(iii) Killed and Attenuated vaccines
(iv) Isograft and Allograft
1x8 = 8
(b) State True or False :
(i) Catalytic monoclonal antibodies are called abzymes.
(ii) IL-12 is a potent activator of macrophages.
(iii) DNA vaccine elicits both humoral and cell mediated responses.
(iv) Bacillus Calmette Guerin (BCG) is an attenuated strain of Mycobacterium bovis.
(v) The increase in CD4+T cells is the hallmark of AIDS.
(vi) Macrophages are the cells which migrate first to the site of inflammation.
(vii) IL-1 and TNF-alpha are known as anti inflammatory cytokines.
(viii) Alfa Feto Protein (AFP) and Carcino Emonyogenic Antigen (CEA) are two well studied oncofetal tumor antigens.
1 P.T.O.
MTBT-302(A)
2. Write short note on the following :
5 x 4 = 20
(a) Antimetabolites
(b) Xenograft
(c) HLA typing
(d) Immunodiagnosis of infections diseases
(a) Describe the method of HAT selection and production of monoclonal antibodies. 15
3.
(b) Give some important clinical uses of monoclonal antibodies. 5
(a) Comment on following : 5x2 = 10
4.
(i) Chimeric toxins
(K) Therapeutic uses of interferons
(b) Give a brief account on general properties of cytokines. Briefly describe biological activity of IL-4 and IL-1'2 cytokines. 10
Write short notes on the following : 5 x 4 = 20
5.
(a) Affinity chromatography for purification of antibodies from blood <b) Routes of antigen administration
(c) Adjuvants
Disadvantages of polyclona. '-mbodies
(a) What are the DNA vaccines? How they are advantageous over
killed and live attenuated vaccines? How DNA vaccines can be
useful to prevent the infection?
15
(b) Comment in brief about immune therapy used for treatment of
5
Asthma
2
Describe sev-eral mechanisms by wmcn iumui uena iu
evade the immune system. 15
Give a brief account of therapeutic strategy currently used against HIV infection. 5
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