Integral University 2010 M.Tech Biotechnology Downstream processing - Question Paper
Roll No. [_ LJ.....j i i
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SECOND SEMESTER EXAMINATION, 2009-10 DOWNSTREAM PROCESSING
Time.
Note
1.
2.
3.
: (i) Attempt any FIVE questions.
(ii) ivlarks are indicated against each question.
Write short notes on the following : 5 x 4 = 20
(a) Adsorption
(b) Foam based Separation
(c)~~ Role of microbes in trickling filters
(d) Gel filtration chromatography
(a) Discuss the mechanisms involved in the adsorption. 10
(b) Write brief note on zone settling and its application in the treatment of wastewater. 10
(a) Discuss the importance of upstream and downstream processing., 10
(b) Explain in detail about the methods involved in extracellular and intracellular product recovery. * 10
Differentiate between : 5 x 4 = 20
(a) Dialysis and osmosis
(b) Discreat settling and Flocculent Settling
*
(c) Micro filtration and Ultra filtration
(d) Adsorption and Absorption
5. (a) Compare the aerobic and anaerobic treatment of wastewater.
Explain about Up-fiow Anaerobic Sludge Blanket Reactor. 10
(b) Derive an expression for flux under concentration polarization for a total retention membrane. 10
Write short notes on the following : 5x2 = 10
6. (a) (b)
(i) Isceiectrofocussing
(ii) Drying
Explain about the gel electrophoresis for the separation of biomolecules. 10
7. Give brief note on the following : 10 x 2 = 20
(a) Super Liquid extraction
(b) High Throughput Screening.
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Earning: Approval pending. |