Integral University 2010 M.Tech Biotechnology Enzyme engineering - Question Paper
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M. Tech. (Biotechnology)
SECOND SEMESTER EXAMINATION, 2009-10 ENZYME ENGINEERING
Time : 3 Hours Total Marks: 100
Note : (i) Attempt any Five questions.
(ii) Marks are indicated against each question.
1. (a) What are the difficulties encountered in isolation and purification
of eukaryotic enzymes in a prokaryotic system? Briefly describe the strategies currently available to increase the yield. 10
(b) Derive the Michelis-Menten equation by steady state of enzyme kinetics. 10
2. (a) What are ailosteric enzymes? How does the kinetics of an
allosteric enzyme differ from that of a conventional enzyme? Justify your answer. 10
(b) What are abzvmes? Discuss their medical and pharmaceutical applications with the help of suitable examples. . 5
(c) What are the factors of a suitable support in an immobilized ' enzyme system? 5
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3. Wii/i ihe help of an example of a su table enzyme describe the kinetics of enzymes catalyzing multisubstrate reactions. 20
4. Describe the methods of immobilization of enzymes and discuss the advantages and disadvantaqes'of each method. - 2C
5. (a) Descf-be "-eta.. iht es:v' r/ ec,-.- j reactor and discuss its
reactor dynamics. What is the effcc;' of non-ideal mixing in an enzyme reactor? 10
(b) What are the indicators of performance of an enzyme reactor? Briefly discuss the factors affecting the reactor performance. 10
6. Present an account of enzyme catalysis in organic media and discuss the utility of enzymes catalyzing such reactions. 20
7. Write short notes on any Four of the following: 5x4 = 20
(a) Inhibition of enzymes
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(b) Analytical applications of enzymes
(c) Mass transfer in enzyme reactors
(d) Ideal enzyme reactor
(e) Rubisco and its significance
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| Earning: Approval pending. |
