Indian Institute of Technology Roorkee (IIT-R) 2009-2nd Year B.Tech Biotechnology Biochemistry and Biophysics - Question Paper

BT 213 BIOCHEMISTRY AND BIOPHYSICS
B.Tech Biotechnology II year Autumn 2009-2010

Summary:
This paper covers 2nd year course on biophysics and biochemistry. It contains ques. on elementary topics like enzymes, metabolic processes in biochemistry and structure of DNA, RNA and proteins in biophysics.

B r 213 BIOCI11-MIS I KY AND BIOPHYSICS B. Tcch. Biotechnology II Year Autumn, 2009-2010

NOTE : Attempt all questions. All Question carry equal marks

Time : 3 hours    M.M. * 40

I. How can you explain helix to coil transitions in polypeptides by molecular mechanism?

b)    Show how experimental results of T,,,/ T\n and dO /dT at T T_m/ do not agree with the simple thermodynamic treatment,

c)    What are the two major shortcomings of simple thermodynamic treatment? How are they removed in Zipper model?

d)    What is the significance of n and s parameters obtained from host-gueM experiment.

e)    What arc the differences and respective advantages to electrophoresis, SDS-PAGE and isoelectric focussing?

I+2+2+1+2=8

2.    a)What are various sugar conformations and which ones exist in native DNA and RNA structures?

b)    Give structural features of A, B. and Z DNA

c)    How does Tm, the melting temperature of DNA depend upon salt concentration and % GC content?

d)    Show Hoogstein and Watson-Criek base pairing schemes. Which one of these more stable?

e)    What is the relative stability of pur-pur, pur-pyr, and pyr-pyr base stacking and why?

2+2+2+1+I=8    j

3.    Give structural features of a helix, |J sheet, (1 turn, 3io and n helix

b)    How do dipolar interactions term contribute to the total energy of polypeptides!? ' What value of dielectric constant should be selected?

c)What    is the basis of Ramachandran map?

d)    Draw <p - y map for Ala. (Jly, Pro, Ala preceding Pro amino acids present in a polypeptide chain.

e)    How do cationic exchange resins differ from anionic resins?

2+2+I+2+1=8

a) What do you understand by photopho.sphorylation? Explain diagrammaticatty hcm ATP and NADPH are generated in light reaction of photosynthesis?

* b) How a fatty acid is transported across the mitochondrial membrane for lipid metabolism? List the reactions of fatty acid catabolism to acetyl CoA in (i-oxidalioo reactions. How many A IP molecules are generated from complete oxidation of a 16-carbon fatty acid to CO; and I IjO?

4+4=8

5. a) Explain how electron transport chain and ATI* synthesis are related in oxidathc phosphorylation? Discuss dhignunmatically the structure of ATP Synthase and proposed mechanism of AIP synthesis by A IP Syntfiavr b) What is the general scheme for cuiuholism of amino acids from different *otirc m liver? Define the terms urcotelic, uricotelic and ammonoicbc.

4+4+8

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Earning:   Approval pending.