Indian Institute of Technology Roorkee (IIT-R) 2009-2nd Year B.Tech Biotechnology Cell and microbiology - Question Paper
Indian Institute of Technology Roorkee
Department of Biotechnology
Course: Cell and micro biology (BT 211)
End term examination/ Autumn semester 2009-2010
Summary:
This ques. paper cover topics on cellular functioning in cell biology and microbes (bacteria, viruses, fungi) in microbiology.
Indian Institute of Technology Roorkee Department of Biotechnology Course: Cell Biology and Microbiology (BT 211)
Kml Term Examination / Autumn Semester 2009-2010
MM : 40 Time : 3 hours
1, Mention two points which arc common for mitochondria and chloroplast. 1
2. What is the application of RFLP in microbiology? What is the basic principle of this method? Explain it with a neat schematic sketch. 3
-3, What do you mean by solid growth media? What is the major material used to make this media at the moment? Prior to this, gelatin was used in making this media but it was withdrawn due to some reasons. What was the reason? 3
4. What do you mean by bioabsorption and how is it different from bioaccumulation? Mention some factors which affects the process of bioabsorption. 3
5. Tatcur's Swan Neck Flask Experiment was one of the pioneering experiments in the field of microbiology. What was the aim and final conclusion of this experiment? 3
6. Microbial world involves various classes of disease causing agents like: protozoa, helminthes, tungi, algae and viruses. Out of the following list of various diseases categorize them into various microbes (as mentioned earlier) which cause them: flu, trichinosis, paralytic shellfish poisoning, malaria, athlets foot, chicken pox. 3
, 7. What do you mean by bioremediation? What are the two basic types of bioremediations are known? Mention the basic difference between them. 3
8. A ligand binds to GPCR which in turn activates G protein, which then activates an enzyme P and that up regulates a second messenger Q. The second messenger in turn activates another enzyme R which actually initiates the cascade of cell signaling. The GPCR is know'n to have S number(s) of transmembrane helices. On the other hand, another ligand passes through the plasma membrane and binds to its receptor in the nucleus or cytoplasm. The unique feature of these receptors is their ability to bind T before giving any response. Due to the biding capacity of these receptors to T these receptors are also known as U. Identify P, Q, R, S, T and U. 3
I. What do you mean by apoptosis? What are the major processes by which apoptosis lakes place. How does it help in organ development? Name some proteins/ enzymes involved in this process. 3
t. Wiese tire the only cells in the body which undergo meiosis. What are these cells called mid why they need to undergo meiosis? ,
Indian Institute of Technology Roorkee Department of Biotechnology Course: Cell Biology and Microbiology (BT 211)
F.ml Term FAaininntion / Autumn Semester 2009-2010
MM : 40 Time : 3 hours
1, Mention two points which arc common for mitochondria and chloroplast. 1
2. What is the application of RFLP in microbiology? What is the basic principle of this method? Kxplain it with a neat schematic sketch. 3
-3, What do you mean by solid growth media? What is the major material used to make this media at the moment? Prior to this, gelatin was used in making this media but it was withdrawn due to some reasons. What was the reason? 3
4. What do you mean by bioabsorption and how is it different from bioaccumulation? Mention some factors which affects the process of bioabsorption. 3
5. Tatcur's Swan Neck Flask Experiment was one of the pioneering experiments in the field of microbiology. What was the aim and final conclusion of this experiment? 3
6. Microbial world involves various classes of disease causing agents like: protozoa, hclniinthes, fungi, algae and viruses. Out of the following list of various diseases categorize them into various microbes (as mentioned earlier) which cause them: flu, trichinosis, paralytic shellfish poisoning, malaria, athlets foot, chicken pox. 3
, 7. What do you mean by bioremediation? What are the two basic types of bioremediations are known? Mention the basic difference between them. 3
8. A ligand binds to GPCR which in turn activates G protein, which then activates an enzyme P and that up regulates a second messenger Q. The second messenger in turn activates another enzyme R which actually initiates the cascade of cell signaling. The GPCR is known to have S number(s) of transmembrane helices. On the other hand, another ligand passes through the plasma membrane and binds to its receptor in the nucleus or cytoplasm. The unique feature of these receptors is their ability to bind T before giving any response. Due to the biding capacity of these receptors to T these receptors are also known as U. Identify P, Q, R, S, T and U. 3
I. What do you mean by apoptosis? What are the major processes by which apoptosis takes place. How does it help in organ development? Name some proteins/ enzymes involved in this process. 3
t. Hiese tire the only cells in the body which undergo meiosis. What are these cells called mid why they need to undergo meiosis? ,
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