Tamil Nadu Dr MGR Medical University 2009 M.Pharm PHARMACEUTICAL BIOTECHNOLOGY IV – ADVANCES IN PHARMACEUTICAL BIOTECHNOLOGY - - Question Paper

MARCH -2009
[KU 333] Sub. Code: 2869
M.PHARM. DEGREE exam
(Regulations 2006)
(Candidates admitted from 2006-2007 onwards)
FIRST YEAR
Branch VI – PHARMACEUTICAL BIOTECHNOLOGY
Paper IV – ADVANCES IN PHARMACEUTICAL BIOTECHNOLOGY
Q.P. Code : 262869
Time : 3 hours Maximum : 100 marks
ans All ques.
I. Essay ques. : (3 X 20 = 60)
1. define different sources of enzymes, their extraction purification and applications. Add a note on the production of glucose isomerase.
2. explain the techniques of genetic engineering. discuss how genes are expressed in E-coli and yeast and the production of vaccines.
3. discuss Hybridoma technology – fusion methods for myeloma cells and R.lymphocytes, selection and screening techniques, production and purification of monoclonal antibodies.
II. Write Short Notes : (8 X five = 40)
1. Production of single cell protein.
2. Techniques of immobilization of enzymes.
3. Site directed mutagenesis.
4. PCR and its applications.
5. Use of data bases in biology.
6. Human genome project.
7. Gene therapy.
8. ? test and chi square test.
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Earning:   Approval pending.