Indian Institute of Technology Kharagpur (IIT-K) 2007 B.Tech Biotechnology Cell
Number of students : 26 Instruction : Answer all questions.
Department of Biotechnology IIT, Kharagpur
Time : 03 hrs. Full Marks : 50 Subject No : BT 23002
Date of Examination : April 24, 2007 (14:00 hrs) End Spring Semester - 2007 Subject: Cell & Molecular Biology
1. (a) What is promoter? (b) How Bacterial RNA polymerase finds its promoter and initiates transcription? (c) Discuss Transcription termination by Rho dependent mechanism (d) How bacterial pre tRNA molecule is processed to mature tRNA?
2 + 4 +2 +2= 10
2. (a)What is an operon ? How repressor regulates lac operon and attenuator regulates Trp operon in E. colil (c) Discuss the regulation of gene expression by steroid hormone in mammalian cells, (d) Which transcription factor is common for all three eukaryotic RNA Polymerase?
1 + 5 + 3 + 1 = 10
3. (a) What are the differences between Prokaryotic and eukaryotic RNA polymeraseas ? (b) What modifications occur at the 5 and 3 end of eukaryotic mRNA immediately after its synthesis? (c) What is spliceosome ? (d) What is the role of Shine- Dalgarno sequence in the initiation of protein synthesis? (e) Which structural motifs are present in the DNA binding domain of eukaryotic transcription factors? (f) What is Wobble position in tRNA amd mRNA ?
2 + 4+1 +1+ 1 +1 = 10
4. No need to write question on the answer script. Write simply question number (s) and its answer (s).
a) The limit of resolution of the light microscope is approximately..........; where
as for TEM is approximately..........
b) The proposed model of membrane structure views as protein are inserted into a lipid bilayer.
in which
.protein form open pores through the membrane, allowing the
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free passage of any molecule of the appropriate size. |
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e) A specialized region of the chromosome that plays a critical role in ensuring the correct distribution of duplicated chromosomes to daughter cells during mitosis is known as..........
f) DNA molecules recombine during meiotic crossing over by.....................
g) In eukaryotic cells the site of protein synthesis is......................
h) Name two microtubule motor proteins (a) ...............; (b).............
i) Ribosomal proteins are imported to the nucleolus from the ................and
begin to assemble on pre-r-RNA prior to its cleavage.
j) 28S, 5.8S and 5S rRNAs are present in....., subunit whereas 18S rRNA is
present in .... subunit.
k) Ribosomal RNA genes are organized around the..............
1) Cytoskeleton is compost of 3 principal types of protein filaments
a)_ b)_c)_
m) A protein which converts chemical energy to mechanical energy for generating
force is called.......................
Dimers of alpha and beta tubulins polymerize to form__
Colchicine blinds to tubulins and inhibits_formation.
ATP binding and hydrolysis play a key role in regulating the assembly and dynamic behavior of.............filaments.
The proteins are targeted to the nucleus by specific amino acid sequences
called..........................that direct the transport through the nuclear pore
complex.
The basic structural unit of chromatin is known as.................
Meiotic crossing over takes place at......................of meiotic prophase I.
The major transitions in the cell cycle are driven by the successive activation of a family................................
n)
o)
P)
r) s) t)
20 x 1-20
2-"*
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| Earning: Approval pending. |
