Thapar University 2006 M.Sc Bio Chemistry Bioanalytical Techniques - Question Paper
Thapar Institute of Engineering & Technology
M.Sc. Biotechnology (1st Year)
Final Term exam
BH006 (Bioanalytical Techniques)
Thapar Institute of Engineering and Technology End semester Examination (BH-0061 Bioanalvtical techniques
13.12.06 Time: 3hour
Max. Marks: 36
Course Instructor: Dr Abhijit Ganguli; Attempt any five question
Q 1. Explain why radioisotopes have assumed significance in biological sciences. Name two widely used radioisotopes.
(5+2.2)
Q2. Describe autoradiography. Give a simple scheme to isolate a commercially important metabolite using radiolabelling
(2.2+5)
Q3. What are high throughput systems? Give examples of their uses in microbial screening and proteomics/genomics. (2.2+5)
Q4. Describe any two methods for disrupting microbial cells.
State one disadvantage of each method. (5.2+2)
Q5.Give an account of Western blotting. Explain briefly how enzymatic activity can be detected over gels. (3.6+3.6)
Q6.Write short notes on (a) Ultra filtration (b) Electron microscopy
(3.6+3.6)
Q7. State Lambert -Beers law. Describe the applications of spectrophotometry in biological sciences giving at least two examples. (2.2+5)
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| Earning: Approval pending. |
