Thapar University 2006 B.Tech Biotechnology Biostatics and Bioinformatics - Question Paper

Thapar Institute of Engineering & Technology
B.Tech. Biotechnology (2nd Year)
Final Term exam
BT009 (Biostatics and Bioinformatics)

THAPAR INSTITUTE OF ENGINEERING AND TECHNOLOGY End-Semester Examination - December 2006 BT-009 BIOSTATISTICS AND BIOINFORMATICS

_Instructor - Dr. N.Tejo Prakash_

Time: 3 Hours    Maximum Marks: 36

The question paper contains two sections (A & B)

__Follow serial order in answering questions_

Section A (Bioinformatics)

ANSWER TWO OUT OF THREE QUESTIONS (2X6 MARKS = 12 MARKS)

1 What are the major tools and extensions of BLAST. Discuss the areas of application of these (6) programs-

2.    Generate Needleman-Wunsch matrix for the following pair of sequences and show the (6) alignment (use score of-2 for gap; +1 for match; and -1 for mismatch).

GGCTTGACCGG

GGATTGACCCG

Differentiate between N W and SW algorithms in terms of their applications *

3.    Describe the application of multiple sequence alignment and differentiate between progressive (6) and iterative methods.

Section B (Biostatistics)

ANSWER THREE OUT OF FOUR QUESTIONS (3X8 MARKS = 24 MARKS)

4.    Using Tukey Test on the following data giving selenium (Se) concentrations (mg/ml) in 5 (8) different groundwater wells, determine if mean Se concentrations are same in all 5 groundwater wells. (F table = 2.75; q ** = 4.16)

Well 1 Well 2 Well 3 Well 4 Well 5 28.2 39.6 46.3 41.0 56.3 33.2 40.8 42.1 44.1 54.1 36.4 37.9 43.5 46.4 59.4 34.6 37.1 48.8 40.2 62.7 29.1 43.6 43.7 38.6 60.0 31.0 42.4 40.1 36.3 57.3

5. Protein content of 5 varieties of leafy vegetables estimated in the five regions is given below. (8) Test the variation due to Regions and Varieties. (Fubte = 2.642)

Varieties	Regionl	Region2	Region3	Region4	Regions
1.	1.82	1.36	0.9	1.20	1.82
2.	1.37	1.18	1.42	2.22	2.08
3.	1.67	1.26	1.34	1.54	1.35
4.	1.64	1.00	1.04	2.62	1.22
5.	1.18	1.72	1.60	0.74	1.03

6. The data of wing length of thirteen sparrows of various ages are given below. Determine whether (8) using lengths are associated with age. Define the regression equation and coefficient of determination. Test the significance of regression using one-way ANOVA (F_tabi_e=4.84)

Age (days) 3 4 5 6 8 9 10 11 12 14 15 16 17 Wing length (cm) 1.4 1.5 2.2 2.4 3.1 3.2 3.2 3.9 4.1 4.7 4.5 5.2 5.0

7. The concentrations of two pollutants were determined in a certain urban area (ng/m3).    (8) (a)    Test the hypothesis that both classes of pollutants were present in the same concentration, using paired sample hypothesis (W - 2.228) (b)    Also test the hypothesis with Wilcoxon paired sample test (Toa* =10)

Day	1	2	3	4	5	6	7	8	9	10	11
Pollutant 1	104	116	84	77	61	84	81	72	61	97	84
Pollutant 2	108	118	89	71	66	83	88	76	68	96	81

Attachment: thapar-university-2006-b-tech-biotechnology-26072-1.pdf

Earning:   Approval pending.