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Thapar University 2006 B.Tech Biotechnology Reaction Engineering & Enzyme Technology - Question Paper

Thursday, 18 April 2013 07:10Web



Thapar Institute of Engineering & Technology
B.Tech Biotechnology (4th Year)
Final Term exam
BT011 (Reaction Engineering & Enzyme Technology)

THAPAR INSTITUTE OF ENGINEERING & TECHNOLOGY, PATIALA

(Department of Biotechnology and Environmental Sciences)

End semester examination (B.Tech Biotechnology IV year)

Course name : Reaction engg. & Enzyme technology Course instructor: G. Vasundhara Time : 3 hr

Course no.: BT 011 Date: 05/12/06 Marks : 36 marks


Attempt any six of the following questions:

1    a) What are the various microenvironmental factors affecting the rate of 4.5

enzyme catalysed reactions? Explain the effect of pH and give the scheme to describe pH dependence of the enzymatic reaction rate for ionizing enzymes.

b) What is the difference between integral and differential method of analysis *-5 of kinetic data?

2    a) What are the various advantages offered by the biocatalytic transformations 3.0

performed in organic media.

b) A substrate is converted to a product by the catalytic action of an enzyme. 3.0 Assume that the Michaelis-Menten kinetic parameters for this enzyme reaction are Km 0.03 mol/1 rmax = 13 mol/1 min. What should bc the size of a steady-state CSTR to convert 95% of incoming substrate (Cso = 10 mol/1) with a flow rate of 10 1/hr?

3    a) Derive the basic performance equation of a CSTR. What will be the 4.0

performance expression for 1st order reaction with constant density systems.

b) Consider a gas-phase reaction 2A 55 R + 2S with unknown kinetics. If a 2.0 space velocity of 1/min is needed for 90% conversion of A in a plug flow reactor, find the corresponding space-time and mean residence time or holding time of fluid in the reactor.

6 Describe the exit age distribution in non-ideal flow reactors? Describe 6.0 systematically how you calculate the conversion based on the information of tracer studies.

7.    Describe the dispersion and tank in series model for predicting the non ideal 6.0 flow behaviors in reactors.

8.    Consider the isothermal gaseous decomposition reaction A3B. The 6.0 laboratory measurements are given in table and show the chemical reaction rate as a function of conversion.

X_-r.\ (mol/l.s)

0.0

0.0053

0.1

0.0052

0.2

0.0050

0.3

0.0045

0.4

0.0040

0.5

0.0033

0.6

0.0025

0.7

0.0018

0.8

0.00125

0.85

0.001

For the two CSTRs in series, 40% conversion is achieved in the first reactor. What is the total volume of the two reactors necessary for 80% overall conversion of the species A entering reactor 1? (If Fa2 is the molar flow rate of A exiting from the last reactor in the sequence, FA2 0.2 FAo)

Fao = 0.867 mol/s. CaVu-AMt He vlu-fr-*    K alImo**

(OMt<Ctcft **' Of* c.







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