Indian Institute of Information Technology 2006 Entrance Exams IIT Entrance Exams JAM BIOTECHNOLOGY TEST - Question Paper

(A) Gel permeation chromatography
(B) SDS-PAGE
(C) Analytical ultracentrifugation
(D) MALDI-TOF mass spectrometry
10. The volume of bacteriophages in a provided sample is best provided as
(A) Colony forming units (CFU)
(B) Minimum inhibitory concentration (MIC) (C) Plaque forming units (PFU)
(D) Lethal dose 50% (LD50)
11. Inter-conversion of glucose and fructose occurs with an equilibrium constant of 1.0.
Glucose isomerase catalyzes this reaction. The final concentration of fructose at equilibrium from 40 mM glucose is
(A) 40 mM (B) 20 mM (C) 10 mM (D) 0 mM
12. Cori cycle integrates body metabolism to
(A) resynthesize glucose from lactate in the liver
(B) oxidize acetyl CoA in the muscle
(C) generate urea in the kidney
(D) generate glucose from acetyl CoA in the liver
13. In a molecule of glycogen we obtain
(A) one reducing end and 1 non-reducing end (B) many reducing ends and 1 non-reducing end (C) one non-reducing end and many branch points (D) one reducing end and many non-reducing ends
14. ABO blood group classification is based on the surface antigen of erythrocytes. The nature of this antigen is
(A) peripheral membrane protein
(B) integral membrane protein
(C) sphingoglycolipid
(D) glycoprotein
15. The citric acid cycle is a metabolic device to oxidize acetyl CoA and conserve the liberated free energy for ATP generation. In this cycle there are
(A) two decarboxylations and 2 oxidations (B) two decarboxylations and 4 oxidations (C) one decarboxylation and 3 oxidations (D) one decarboxylation and 1 oxidation

16. In an enzyme catalyzed reaction, doubling the amount of enzyme leads to a change in which of the following?
(A) Vmax
(B) kcat
(C) kcat/Km
(D) Km
17. Growing E. coli cells, when shifted from 37?C to 20?C, maintain their membrane fluidity by modifying the subsequent parameters
(A) Fatty acid chain length and sterol content (B) Fatty acid chain length and unsaturation (C) Fatty acid unsaturation and sterol content (D) E. coli cannot maintain membrane fluidity

Earning:   Approval pending.