Indian Institute of Information Technology 2006 Entrance Exams IIT Entrance Exams JAM BIOTECHNOLOGY TEST - Question Paper

JAM 2006



BIOTECHNOLOGY TEST PAPER

1. Lysine is an amino acid with 3 ionizable groups. These are the a-COOH, a-amino and ?-amino groups with pKa values of 2.2, 9.2 and 10.8, respectively. The isoelectric point (pI) for lysine is

(A) 5.7
(B) 6.5
(C) 9.2
(D) 10.0
2. Which of the subsequent pair of vitamins participates as cofactors in the cellular oxidation- reduction reactions?
(A) Pyridoxine and folate
(B) Riboflavin and cobalamine (C) Ascorbate and nicotinamide (D) Thiamine and pantothenate
3. Erythrocyte glucose transporter specifically transports glucose down its concentration
gradient and exhibits hyperbolic saturation kinetics. This is an example of
(A) Active mediated transport (B) Passive mediated transport (C) Non-mediated transport (D) Group translocation
4. The oligosaccharide part of glycoproteins is either N-linked or O-linked. The amino acid residues through which these oligosaccharides are attached to polypeptide are
(A) Asn and Thr (B) Asp and Ser (C) Ser and Lys (D) Gln and Ser
5. select the right combination of components needed to set up a polymerase chain reaction from the subsequent
(A) Template DNA, 2 primers, dNTPs and DNA ligase
(B) Template DNA, 2 primers, NTPs and DNA ligase
(C) Template RNA, 2 primers, NTPs and DNA polymerase
(D) Template DNA, 2 primers, dNTPs and DNA polymerase

6. The DNA restriction site recognized by the enzyme Hind III is – AAGCTT . Which of the
TTCGAA
subsequent sequence contained in a double stranded DNA is cut by Hind III?




(A) Only Q

(P) (R)

ATGCCTTAAGATGC ATGCAAGCTTATGC

(Q) (S)

ATGCTTCGAAATGC ATGCGAATTCATGC

(B) Both Q and R (C) Only R
(D) All of them
7. A bacterial culture contained 32x106 cells after 2.5 hours of exponential growth. If the doubling time was 30 min, what was the initial population number in this culture?
(A) 20x104 cells (B) 10x105 cells (C) 40x105 cells
(D) 16x106 cells

8. The accurate match ranging from Group I and Group II is

Group I Group II
P. Amphotericin B 1. Reverse transcriptase inhibition
Q. Azidothymidine (AZT) 2. Sterol binding and disruption of membrane
permeability
R Nalidixic acid 3. Blocks glycosylation
S. Tunicamycin 4. DNA gyrase inhibition
(A) P-2, Q-1, R-4, S-3
(B) P-1, Q-4, R-3, S-2
(C) P-4, Q-1, R-3, S-2
(D) P-3, Q-2, R-4, S-1
9. Most right method to determine the molecular weight of a provided polypeptide is

Earning:   Approval pending.