Punjab Technical University 2007 B.Sc Biotechnology ENZYMOLOGY

Paper ID [C1508]
(Please fill this Paper ID in OMR Sheet)

B.Sc. BT (202) (S05) / 102 (Old) (Sem. - 2nd)

ENZYMOLOGY & IMMUNOLOGY
Time : 03 Hours Maximum Marks : 75
Instruction to Candidates:
1) part - A is Compulsory.
2) Attempt any 9 ques. from part - B.
part - A
(15 x two = 30)
Q1)
a) describe enzymes.
b) discuss the cause that all enzymes are proteins but not all proteins are
enzymes.
c) How the enzyme activities are measured?
d) What do you understand by reversible inhibition of the enzyme?
e) discuss the cause for the specificity of the enzyme reactions.
f) elaborate the various factors that affect the activities of the enzymes?
g) provide examples of the substrate analogous of the enzymes and their uses.
h) elaborate the various strategies used to increase the storage stability of
the enzyme?
i) describe coenzyme, cofactor.
j) Name the various kinds of sources of the enzymes.
k) What do you understand by inhibition of the enzyme?
l) How the name of enzymes are assigned?
m) What do you understand by coenzymes and metallo enzyes? provide
examples.
J-8224[S-9700199] P.T.O.
n) Name a few of the largest producing enzymes used commercially.
o) What do understand by zymogens? What is the importance of the
zymogens?
part - B
(9 x five = 45)
Q2) elaborate the assumptions in the enzyme substrate reactions by Michaelis-
Menten? Derive the Michaelis-Menten kinetics rate expression of the enzyme
reactions.
Q3) Name a few enzymes and their sources used as medicine. define their
applications.
Q4) explain about the industrial applications of the enzymes for the production of
liquid glucose from starch.
Q5) Name and explain about the various theories of the enzyme substrate
interaction in enzyme catalyzed reactions.
Q6) explain about the application of the enzymes in the beer industries.
Q7) explain about the application of enzymes in detergent and textile industries.
Q8) explain about the different graphical methods for the measurement of kinetic
parameters from the rate equations of the enzyme reaction.
Q9) elaborate the various techniques used in the immobilization of the enzymes?
explain about technique of immobilization of covalent method.
Q10)Discuss about the effect of pH on the activities of the enzymes.
Q11)What is the various methods for separation of enzymes? discuss the
sequential steps for the separation of the enzymes from fermentation broth.
Q12)Discuss about the diagnostic applications of the enzymes.
Q13)Why the concentration of enzyme is expressed in

Earning:   Approval pending.