Punjab Technical University 2010-4th Sem B.Sc BioInformatics .(BI) (th) MOLECULAR GENETICS - Question Paper
[Total No. of Pages : 02
Roll No.......................
Total No. of Questions : 13]
[2037] B.Sc.(BI) (Semester - 4th)
J-3454[S-1310]
MOLECULAR GENETICS (B.Sc.(BI)-401)
Maximum Marks : 75
Time : 03 Hours
Instruction to Candidates:
1) Section - A is compulsory.
2) Attempt any Nine questions from Section - B.
Section - A
(15 x 2 = 30)
Q1)
a) Classify the different nucleic acid bases.
b) Define nucleosides and the nucleotides.
c) What are the results of the X-ray diffraction of the DNA.
d) Explain the overlapping gene.
e) What is operon?
f) What do you understand by central dogma.
g) Explain the term DNA sequencing and its utility.
h) Explain the transposon.
i) What is genomic library. What is its utility? j) Define the term reading frame.
k) What are the restriction enzymes and their sources?
l) What do you understand by gene mapping?
m) What is the basic principle of the jell electrophoresis? What is its use?
n) What do you understand by genetic clone?
Q2) Differentiate between the replication and transcription. Discuss the enzymes involved in the replication of the DNA.
Q3) Discuss about the structure of the DNA.
Q4) What do you understand by translation. Discuss about the important characteristics of the genetic codes.
Q5) Show and explain the principal steps of the bacterial transformation.
Q6) Write short note (any one)
(a) PCR
(b) Chromosome replication cycle.
Q7) What are the different levels of gene expression and mechanisms of their regulations involved.
Q8) Describe the principle of DNA fingerprinting.
Q9) What do you understand the repetitive DNA. Describe different variations in the sequence architecture of the repetitive DNA.
Q10)Discuss about the construction of genomic library of Eukaryotic organism.
Q11)Name the different techniques of the recombinant selection. Discuss about the recombinant selection by hybridization.
Q12)What are the different types of genetic recombination takes place in bacteria. Discuss about the general mechanisms.
Q13)Classify different types of vectors. Discuss about the vector phage virus.
J-3454[S-1310] 2
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