Jaypee Institute of Information Technology (JIIT) 2008 B.E Test 3 : Industrial Plant Tissue Culture - Question Paper
JYPEE UN1VERISTY OF INFORMATION TECHNOLOGY WAKANGHAT SOLAN Test 3 Nov. 2008 B. Tech VHth Semester (BT, BI)
Course code :07B71BT433 Max. Mark 30
Course Name: Industrial Plant Tissue culture Max. Time 90 Mid.
Note: First question is compulsory. Do any three out of others.
Q.l Answer following questions briefly. 6X2=12
1. Why il is advised to have a two stage production system for secondary metabolite production through plant cell cultures?
2. What type of variations can be exploited for selection of cell line for high secondary metabolite production?
3. Why cell suspension culture of Lythospermum eryihrorhi:on does not produce shikonin when irradiated with light?
4. Chemically defined elicitors being used for elicitation of secondary metabolites production stimulate pathogenesis related defense mechanism in plants. Justify by citing suitable example.
5. Why forced ventilation will be more beneficial as compared to natural ventilation in commercial PAM?
6. The microbial contamination in PAM is reduced as compared to PMM. Justify?
Q.2 WTiat is autotrophic micro propagation? Enlist three most important conditions to be met to achicvc autotrophic growth of plants in vitro. Elaborate advantages of autotrophic micro propagation with spccial emphasis on how these enhance the efficiency of micro propagation process and reduce the cost of production.
Q.3 What do you understand by primary and secondary metabolites? Enumerate general functions of secondary metabolites. Why plant cell and tissue culture tcchniquc are being investigated for production of secondary metabolites?
6
Q.4 What do you understand by elicitation and elicitors? Discuss elicitor induced cffccts on plant cells and classification of elicitors giving suitable examples.
6
Q.5 Give a list of important phytochemicals which are potential targets to be produced by plant ceil culture. Discuss antitumor or food additive pigments in details.
6
Q.6 What is the basis of variability of high product producing cell lines selection? Discuss available methods for differentiating producer and non producer cells and cell line selection procedure. 6
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