Jaypee Institute of Information Technology (JIIT) 2008 B.E Test 3 : Protein Engineering and Applications - Question Paper

JAYPEE UNIVERSITY OF INFORMATION TECHNOLOGY TEST -III ( NOVEMBER 2008)

B.TECH (SEMESTER VII)

COURSE CODE: 07B81BT426    MAX MARKS: 30

COURSE NAME: PROTEIN ENGINEERING & APPLICATIONS

COURSE CREDIT: 3    MAX TIME: IHR 30 MIN

Ql) Decreasing the number of unfolded conformations of a protein can help stabilize its native state. Justify the statement with examples.    (6)

Q2) Attachment of SUMO to N-terminus of under-expressed proteins dramatically enhances their expression in E.coii. Discuss in details.    (6)

Q3) You are given a protein X' (MW 36 kDa). How can it be engineered using combinatorial method to increase its stability?    (6)

Q4) Properties of a protein (say an enzyme) can be altered to improve it by mutating the gene coding for tlie proteins using site-directed mutagenesis'. Comment.    (6)

Q5) Differentiate between the following:

i)    Random mutagenesis by dNTP analogs & Random mutagenesis by error-prone PCR

ii)    Protein engineering & Protein design    (6)