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Jaypee Institute of Information Technology (JIIT) 2008 B.E Test 1 : Industrial Enzymes - Question Paper

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JAYPEE UNIVERSITY OF INFORMATION TECHNOLOGY

WAKNAGHAT. SOLAN

TEST - I FEB 2008 SEMESTER VIII

Course Name: Industrial Enzymes    Max Marks 20

Course Code. 07B81BT421    Max Time Ihr

Course credit: 3

AH Questions are compulsory

Ql A pure enzyme (20ug) catalyses (he conversion of 30 umoles of substrate 'X' to

product Y in 10 minutes

Calculate its catalytic activity in terms of

i)    Enzyme unit (U)

ii)    SI unit ( Ratal)

Also calculate the specific activity of the enzyme. (5)

Q2) Regulatory enzymes do not obey Michaelis-Menten kinetics; these enzymes typically

give velocity versus substrate curves that are sigmoidal rather than hyperbolic

What is the significance of this change in shape from hypeitolic to sigmoidal ? Explain

(5)

Q3) If the pi of an enzyme (from ProtParam) is 9 0 and molecular weight 65kDa How will you purify this enzyme?

Make a flow chart to depict the various purification steps    (6)

Q4) Explain the effects of a competitive inhibitor and a non-competitive inhibitor on reaction rate as a function of substrate concentration (4)







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