Jaypee Institute of Information Technology (JIIT) 2008 B.E Test 1 : Industrial Enzymes - Question Paper
JAYPEE UNIVERSITY OF INFORMATION TECHNOLOGY
WAKNAGHAT. SOLAN
TEST - I FEB 2008 SEMESTER VIII
Course Name: Industrial Enzymes Max Marks 20
Course Code. 07B81BT421 Max Time Ihr
Course credit: 3
AH Questions are compulsory
Ql A pure enzyme (20ug) catalyses (he conversion of 30 umoles of substrate 'X' to
product Y in 10 minutes
Calculate its catalytic activity in terms of
i) Enzyme unit (U)
ii) SI unit ( Ratal)
Also calculate the specific activity of the enzyme. (5)
Q2) Regulatory enzymes do not obey Michaelis-Menten kinetics; these enzymes typically
give velocity versus substrate curves that are sigmoidal rather than hyperbolic
What is the significance of this change in shape from hypeitolic to sigmoidal ? Explain
Q3) If the pi of an enzyme (from ProtParam) is 9 0 and molecular weight 65kDa How will you purify this enzyme?
Make a flow chart to depict the various purification steps (6)
Q4) Explain the effects of a competitive inhibitor and a non-competitive inhibitor on reaction rate as a function of substrate concentration (4)
|
Attachment: |
| Earning: Approval pending. |
