Uttar Pradesh Technical University (UPTU) 2010 B.Pharm Pharmaceutical Biotechnology - Question Paper

B. Pharm (UPTU)
Eighth Semester Examination, 2009-10
Pharmaceutical Biotechnology

Time: three Hours Total Marks: 80

Note: 1) Attempt All ques..
2) Each ques. carries equal marks.

1) Attempt any 4 of the following: (4×4=16)
a) Compare antigen and antibody containing preparations.
b) Give official standards of BCG.
c) Define haptens and discuss its role as carrier system.
d) Discuss humoral immunity in brief.
e) Write short note on Null cell (NK cell).

2) Attempt any 2 of the following: (2×8=16)
a) Give formulation process and quality control of monoclonal antibodies formulations and discuss its role in drug targeting.
b) Define protoplast fusion. provide different techniques of protoplast fusion and discuss its role in hybridization.
c) Write short notes on any Two:
i) Humulin
ii) Humatrope
iii) Transformation

3) Attempt any 2 of the following: (2×8=16)
a) Give principle of selection and design of fermentors. Enumerate the characteristics of microorganisms to be used in it.
b) Discuss different methods for standardization of antimicrobials.
c) Write notes on any 2 of the following:
i) Factors affecting mutation
ii) Screening of soil
iii) Historical development of antibiotics

4) Attempt any 2 of the following: (2×8=16)
a) Describe different kinds of reactions mediated by microorganisms.
b) Give biotransformation process for steroids.
c) Write a brief review on pharmaceutical applications of microbial transformation.

5) Attempt any 2 of the following: (2×8=16)
a) Discuss reverse miceller and emulsion method for enzyme immobilization.
b) Give method for the entrapment of plant cell in chitosan and beaded polymers.
c) Write notes on any 2 of the following:
i) Penicillinase
ii) Amylases
iii) Streptokinase

Earning:   Approval pending.