Uttar Pradesh Technical University (UPTU) 2010 B.Pharm Pharmaceutical Microbiology - Question Paper

B. Pharm (UPTU)
Fourth Semester Examination, 2009-10
Pharmaceutical Microbiology

Time: three Hours Total Marks: 80
Note: 1) Attempt All ques..
2) Each ques. carries equal marks.

1) Answer any 4 of the following: (4×4=16)
a) Give pharmaceutical importance of microbiology with suitable examples.
b) Draw a neat and tagged diagram of gram-negative bacterial cell.
c) Define terms – Terca and Bergey's Manual.
d) Explain any 1 method for classification of Bacteria.
e) Discuss the function of Capsule and Flagella.

2) Answer any 4 of the following: (4×4=16)
a) Define subsequent terms:
i) Stain
ii) Smear
iii) Fixation
iv) Acid fastness
b) Give method for gram-staining and discuss its significance.
c) Explain preparation method of "Nutrient agar media".
d) Discuss different isolation methods for virus.
e) Write briefly on "electron microscopy".

3) Answer any 4 of the following: (4×4=16)
a) Describe the following:
i) Sterilization
ii) Disinfectant
b) Discuss the dilution test for the valuation of disinfectant.
c) Give method for sterilization of thermolabile materials.
d) Discuss physical, chemical and biological indicators for validation of sterilization process.
e) Write brief note on 'D Value'.

4) Answer any 2 of the following: (2×8=16)
a) Discuss Sterility testing as per I.P.
b) Explain effectiveness of antimicrobial preservatives.
c) Write short note on personal microbiology.

5) Answer any 2 of the following: (2×8=16)
a) What is 100 class clean area? provide particulate matter test as per I.P.
b) Give Test organism and assay method for Amphotericin B and Tetracycline.
c) Give an overview of Environmental Microbiology.

Earning:   Approval pending.