Uttar Pradesh Technical University (UPTU) 2010 B.Pharm Pharmaceutical Analysis-II - Question Paper

B. Pharm (UPTU)
Fourth Semester Examination, 2009-10
Pharmaceutical Analysis-II

Time: three Hours Total Marks: 80
Note: 1) Attempt All ques..
2) Each ques. carries equal marks.

1) Answer any 4 of the following: (4×4=16)
a) Discuss the importance of non-aqueous titrations in pharmaceutical analysis.
b) What do you understand by levelling effect? How does acetic acid current in acetone-perchloric acid behaves as a base? discuss.
c) How will you prepare 1L of 0.1M HClO4 solution?
d) Write a short note on pM indicator. provide specific example to justify your ans.
e) Give the basic principle of complexometric titrations. discuss the effect of pH on complexation.

2) Answer any 4 of the following: (4×4=16)
a) Give a brief account on the stability of Karl-Fischer reagent and limitations of Karl-Fischer titration.
b) Discuss the standardization of 0.1M NaNO2 solution.
c) Give the merits and demerits of Radio-immuno-assay.
d) Discuss the principle of alcohol estimation in galenicals.
e) Briefly write on nitrogen estimation by Kjeldahl method.

3) Answer any 4 of the following: (4×4=16)
a) Write in short on reference electrodes. provide examples.
b) Give instrumentation of potentiometry.
c) Give pharmaceutical applications of conductometry.
d) Define the following:
i) Electrode potential
ii) Salt bridge
iii) Standard potential
iv) Dielectric cell
e) Derive Nernst formula for measuring the potential.

4) Attempt any 4 of the following: (4×4=16)
a) "Thin layer chromatography is superior to other kinds of chromatographic methods." discuss.
b) What are the various methods for the preparation of chromatoplates?
c) Discuss the different kinds of paper chromatography.
d) Discuss the various factors affecting column efficiency.
e) Enlist pharmaceutical applications of chromatography.

5) Attempt any 2 of the following: (2×8=16)
a) Sketch the component of gas chromatography and define different detectors used in it.
b) What is the principle of HPLC? provide the comparison of HPLC and GLC.
c) Write an exhaustive note on column packing in HPLC. explain a few commercially available packing material and their characteristics.

Earning:   Approval pending.