Bharathiar University 2011-2nd Sem M.Sc BioTechnology GENETIC ENGINEERING - Question Paper

Time: 3 hours

Maximum:75 marks

ans all ques.

part A - (10 X one = 10 MARKS)

1. Affinity chromatography

2. Eco RI

3. Genetic linkage analysis

4. Lytic phase

5. Pro-phase

6. Fusion vectors

7. PBR322

8. Systemic lupus erythromatus

9. Cosmids

10. Chromosomal walking

part B - (5 X five = 25 MARKS)

11.(a)Enumerate the principle, procedure and applications of Southern blotting.
Or
(b)Importance of Agarose gel electrophoresis in the field of Biological research.

12.(a)Explain in detail the biological applications of lambda phage and yeast vectors.
Or
(b)Applications of finger print analysis in Forensic science.

13.(a)What are special vectors? discuss with any 2 examples.
Or
(b)Homologous recombinant (2 1/2)

Non homologous recombinant (2 1/2)

14.(a)Which kind of mutation can be detected using SSCP? explain with example.
Or
(b)Write short notes on protein engineering and its applications.

15.(a)Give the significance of Human Genome Project.
Or
(b)Give the merits and demerits in latest patterns on genetic engineering.

part C - (5 X eight = 40 marks)

16.(a)Discuss on the different steps involved in recombinant DNA technology.
Or
(b)Write the principle, procedure and applications of northern blotting.

17.(a)Describe in detail the 2 major techniques involved in the DNA sequencing.
Or
(b)Codon optimization in designing vectors. (4)

Characteristic feature of vector that could carry plant genetic material.(4)

18.(a)Give detailed protocol in constructing cDNA library.
Or
(b)Discuss about the applications of RFLP in different fields of Biological research.

19.(a)Write notes on the significance of hetero duplex analysis of DNA in the detection of
mutations.
Or
(b)What are the steps involved int he stabilization of recombinant proteins?

20.(a)Give a detailed account on he latest advancements in the gene therapy with an example.
Or
(b)Discuss on how gene therapy is successfully employed in treating:

Cystic fibrosis

Lesch nyphan syndrome.

Earning:   Approval pending.