Bangalore University 2009-2nd Sem B.Sc Biotechnology Iester , / - Question Paper

III sem bsc. exam , nov/dec 2009.

BIOTECHNOLOGY -III

BIOCHEMISTRY AND BIOPHYSICS.

instructions:

I) part I and part II must be answered in seperate booklets.
II) draw the structures and labelled diagrams wherever necessary.

PART I
(biochemistry)
part A

I) ans any 4 of the subsequent :- (4*2=8)

1) peptide chain.
2) thiamine.
3) zwitter ion.
4) saponification.
5) coenzyme.

part B

II) ans any 2 of the following:- (2*6=12)

6) discuss the D and L forms of carbohydrates with example.
7) provide an account on properties of amino acids.
8) discuss IUB system of enzyme classification.

sction C

III) ans any 2 of the following:- (2*10=20)

9) write the dietary source and clinical significance of vitamin B complex.
10) provide the classification of proteins based on structure and function.
11) discuss the effect of pH and substrate concentration of an enzyme activity.
12) define the various kinds of fatty acids with examples.

part II
(biophysics)
part A

I) ans any 2 of the following:- (2*5=10)

1) provide a note on hydrogen bond and peptide bond.
2) define the measurement of radioactivity by scintallation counter.
3) write the principle and applications of ultracentrifugation.

part B

II) ans any 1 of the following:- (1*10=10)

4) discuss the laws of thermodynamics and their limitations.
5) provide abrief account on paper chromatography.

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Earning:   Approval pending.