Birla Institute of Technology (BIT Mesra) 2007 B.E Recombinant DNA Technology - Question Paper
Birla Institute of Technology and Science ,Pilani.
1st Semester 2007-2008
Comprehensive exam BioC461 Recombinant DNA Technology
Mmarks 30 Time 3h
1)Is DNA translocation needed in enzymes used in Rec DNA. discuss logically ?3
2) How would you join fragments of DNA made with the enzymes Hind11 and EcoR1 ? 3
3) Are Sau3A1 and BamH1 isochizomers, what is the characteristics of the product found on joining these two enzyme digests ? 3
4) In a real time PCR experiment no upregulation of signal was seen although the gene was supposed to be upregulated in microarray experimentation. What could be issues in the components of the Real time PCR reaction ? 3
5) In an experiment involving cloning and expression of an eukaryotic gene in a prokaryotic vector system on signal was seen on Western blot. elaborate the steps you would take as an experimentalist to trouble shoot this issue ? 6
6) What would happen in a lambda phage infection if (4x2)
a) The sigma 70 Ecoli RNA pol underwent a loss of function mutation
b) The qut sites were mutated
c) Sib was deleted
d) CI was supressed
7) What happens in the GEP if the signal peptidase cleavage site is mutated ? 2
8) Can liposome mediated transfection used for plant cells ? discuss 2
Birla Institute of Technology and Science ,Pilani.
1st Semester 2007-2008
Comprehensive Examination BioC461 Recombinant DNA Technology
Mmarks 30 Time 3h
1)Is DNA translocation required in enzymes used in Rec DNA. Explain logically ?3
2) How would you join fragments of DNA made with the enzymes Hind11 and EcoR1 ? 3
3) Are Sau3A1 and BamH1 isochizomers, what is the characteristics of the product obtained on joining these 2 enzyme digests ? 3
4) In a real time PCR experiment no upregulation of signal was seen although the gene was supposed to be upregulated in microarray experimentation. What could be problems in the components of the Real time PCR reaction ? 3
5) In an experiment involving cloning and expression of an eukaryotic gene in a prokaryotic vector system on signal was seen on Western blot. What are the steps you would take as an experimentalist to trouble shoot this problem ? 6
6) What would happen in a lambda phage infection if (4x2)
a) The sigma 70 Ecoli RNA pol underwent a loss of function mutation
b) The qut sites were mutated
c) Sib was deleted
d) CI was supressed
7) What happens in the GEP if the signal peptidase cleavage site is mutated ? 2
8) Can liposome mediated transfection used for plant cells ? Explain 2
| Earning: Approval pending. |
