Bhavnagar University 2007 M.Phil Zoology Research Methodology and Laboratory Technques - Question Paper

M.Phil. DEGREE EXAMINATION, MAY 2007
Non–Semester
Zoology
RESEARCH METHODOLOGY AND LABORATORY TECHNIQUES

Time : 3 hours Maximum : 100 marks
ans any 5 ques..
All ques. carry equal marks.
1. Give an account on basic and working principles of scanning and transmission electron microscope. Add a note on their applications in biological sciences. (20)
2. (a) What is fluorescence? discuss the basic and working principle of microscope. (8)
(b) What is microtechniques? discuss the process of cryostat and ultra microtome sectioning and staining of biological materials. (12)
3. (a) What is pH? provide a brief account on basic principles of pH determination using pH meter. Add a note on glass electrode. (12)
(b) Give a detailed account on X-ray diffraction and its application in structural studies. (8)
4. What is electrophoresis? discuss the uses of SDS-PAGE in quantitative analysis of proteins. (20)
5. What is chromatography? explain the principles and applications of HPTLC and affinity chromatography. (20)
6. (a) What are the basic principles involved in the isoelectric focusing and its significances. (10)
(b) What is monoclonal antibodies and write a essay an hybridoma technology. (10)
7. What is blotting techniques? explain the procedure and application of southern and northern blotting. (20)
8. Give a short notes on the subsequent : (20)
(a) Types of data bases
(b) Protein motifs
(c) Primer designing
(d) Plasmid mapping.

Earning:   Approval pending.