Anna University Chennai 2010-4th Sem B.Tech Biotechnology B.e./, il/ ,ester,Microbiology - Question Paper
B.e./b.tech.degree examination, april/may 2010,fourth semester,Microbiology
B.E./B.Tech. DEGREE EXAMINATION, APRIL/MAY 2010 Fourth Semester Biotechnology BT2255 MICROBIOLOGY (Regulation 2008)
,--V* V/1I
Time: Three hours Maximum: 100 Marks
Answer ALL Questions PART A (10x2 : 20 Marks)
1. What is refractive index? Give its importance in microbiology.
2. Give an account on Kochs postulate.
3. What are endospores? Give example.
4. Differentiate the cell wall composition of Gr&m positive and negative bacteria.
5. What is simple and differential staining?
6. What are facultative anaerobic organisms? Give one example.
7. Define bacteriostasis and bactericidal.
8. Comment on anti-viral chemical agents with example.
9. Give an account on bioleaching.
10. What are secondary metabolites? Give its significance.
I V ! PART B (5 x 16 = 80 Marks)
11. (a) Explain the methods of Gram staining and acid fast staining in detail.
Or
(b) Explain the working principle of SEM and TEM with neat labeled diagram.
12. (a) Describe the lytic and lysogenic life cycle of bacteriophage. Give two
examples for bacteriophage.
Or
(b) Discuss the classification and reproduction of fungi in detail.
13. (a) Detail the nutritional requirement of bacterial growth in culture.
Or .
(b) Describe TCA cycle in detail along with its energetic and its significance for the production of energy in bacteria.
14. (a) Describe the mode of action of penicillin and sulfa drugs in detail.
Or
(b) Explain the characteristics of antimicrobial agents and discuss briefly about antibiotic resistance.
15. (a) Describe in detail the industrial production of Vitamin B12 using
microorganisms. j j
%
Ur
(b) Brief about bio pesticides; bio preservatives and bio fertilizers with suitable examples for each.
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