Anna University Chennai 2010-4th Sem B.Tech Biotechnology B.e./, il/ ester, instrumental method of analysis - Question Paper
B.e./b.tech.degree examination, april/may 4th semester, instrumental method of analysis
Reg. No. :Je> 6 O f" Z t f O 2- ]
B.E./B.Tech. DEGREE EXAMINATION, APRIL/MAY 2010 Fourth Semester Biotechnology
BT2254 INSTRUMENTAL METHODS OF ANALYSIS (Regulation 2008)
Time: Three hours Maximum: 100 Marks
Answer ALL Questions
PART A (10 x 2 = 20 Marks)
1. Explain, what is meant by significant figures?
2. What type of noise can be used for reduction of frequency?
3. Explain the term chromophore and give examples.
4. What is the relation between wavelength and energy of electromagnetic radiations?
5. What is Rayleigh scattering?
6. Define transmittance.
7. Name the factors, which affect the thermogravimetric curve.
8. JSxplain how DTA differs from TGA?
s.- . '
$. jWrite short notes on electrophoresis.
10. Explain, what is meant by Rf value?
PART B (5 x 16 = 80 Marks)
11. (a) Enumerate the software techniques used for signal to rioise
enhancement.
Or
(b) Describe what are the types of errors encountered in analytical measurement?
12. (a) List out the advantages of fast Fourier transformation over ensemble
averaging as a signal enhancement technique.
Or
(b) Give brief answers:
(i) Wave length sectors
(ii) Radiation transducers.
13. (a) Explain the various components of UV spectrophotometer.
Or
(b) Discuss the various applications of IR spectroscopy in analytical field.
14. (a) Draw the block diagram of the instrument DTA. Discuss DTA
thermogram of calcium acetate monohydrate.
Or
(b) Discuss the applications and merits of thermogravimetric analysis.
15. (a) Describe the general principle underlying the electrophoresis method for
separation of mixture.
Or
(b) Discuss the principle and applications of ion exchange chromatography.
PART B (5 x 16 = 80 Marks)
11. (a) Enumerate the software techniques used for signal to rioise
enhancement.
Or
(b) Describe what are the types of errors encountered in analytical measurement?
12. (a) List out the advantages of fast Fourier transformation over ensemble
averaging as a signal enhancement technique.
Or
(b) Give brief answers:
(i) Wave length sectors
(ii) Radiation transducers.
13. (a) Explain the various components of UV spectrophotometer.
Or
(b) Discuss the various applications of IR spectroscopy in analytical field.
14. (a) Draw the block diagram of the instrument DTA. Discuss DTA
thermogram of calcium acetate monohydrate.
Or
(b) Discuss the applications and merits of thermogravimetric analysis.
15. (a) Describe the general principle underlying the electrophoresis method for
separation of mixture.
Or
(b) Discuss the principle and applications of ion exchange chromatography.
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