Berhampur University 2009 M.Sc BioTechnology ENZYMOLOGY - Question Paper

1.Describe the steps for the purification of enzyme

2.Describe the mechanism of reaction catalysed by chymotrypsin.

3.Write notes on
a.Alosteric control of enzymes

b.General acid-base catalysis.

4.Derive Michaelis-Menten formula. Add a note on the significance of Km value.

5.Write notes on
a.Competitive inhibition of enzyme

b.Factors influencing enzyme kinetics

6.Describe the determination of 3-D structure of enzyme active site.

7.Write notes on the following:

a.Use of substrates analogues for the determination of active site of enzymes.

b.Enzyme catalysis in solution.

8.describe the physical methods of immobilization of enzymes and add a note on the industrial application of immoblized enzymes.

Earning:   Approval pending.