M.Sc-M.Sc Bio Chemistry 2nd Sem BCH - 271 : Techniques for Characterization of biomolecules(University of Pune, Pune-2013)

                           M.Sc. (Semester - II)

                            BIOCHEMISTRY

SEAT No. :

[Total No. of Pages : 2

BCH - 271 : Techniques for Characterization of biomolecules

(2010 Pattern)

Time : 3 Hours]                                                                                           Max  marks :80

Instructions to the candidates :

1) All questions are compulsory.

2) Figures to the right side indicate full marks.

Q1) Attempt any four of the following :

Distinguish between boundary and band sedimentation.

How sensitivity of autoradiography is achieved?

Draw the schematic diagram of NMR and explain the instrumentation.

What is polarization of fluorescence? List the basic rules for interpretation.

Explain the special uses of LCMS in biology and biochemistry.

Q2) Attempt any four of the following :                                                                [20]

a)       Describe the applications of ESR.

b)       Define and give application of partial specific volume. How it is determined

by pycnometer?

c)       What is the effect of addition of ethidium bromide on viscosity of DNA.

d)       Explain the types of radiations used in Biochemistry.

e)       Explain the relationship between electrical response and rate of diffusive

flux.

P.T.O.

1

Q3) Answer any two of the following :                                                                  [20]

Give the principle and application of IR spectroscopy.

Explain the major applications of biosensors in environmental pollution

monitoring.

Describe briefly the theory of atomic absorption spectroscopy. what

information can be obtained from this spectroscopy? Explain any one application.

Q4) Write short notes : (any four)                                                                            [20]

X-ray diffraction. ORD and CD.

MALDI- TOF-MS. Immunosensor.

Autoradiography.

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