M.Sc-M.Sc Bio Chemistry 1st Sem BCH - 171 : Enzymology and Biophysical Techniques(University of Pune, Pune-2013)

                           M.Sc. (Semester - I)

                                BIOCHEMISTRY

SEAT No. :

[Total No. of Pages : 2

BCH - 171 : Enzymology and Biophysical Techniques

(2010 Pattern)

Time : 3 Hours]                                                                                               [Max. Marks : 80

Instructions to the candidates :

1) All questions are compulsory.

2) Answers to both the sections should be written in separate answer books.

3) Figures to the right indicate full marks.

SECTION - I

(Enzymolgy)

Q1) Answer any three of the following :                                                                [15]

a) What is substrate cycle? Explain with suitable example.

b) Discuss and two factors leading to rate enhancement of enzyme catalyzed

reactions.

c) Explain the mechanism of action of triosephosphate isomerase.

d) Define the terms :

i)        apoenzyme                     ii) holoenzyme

iii) allosteric site                  iv) cofactor

Q2) Answer any three of the following :                                                                [15]

a) Discuss in detail effect of substrate concentration on enzyme catalyzed

reaction.

b) Explain the various conditions under which the enzyme-substrate complex

is stabilized to determine the mechanism of enzyme catalysis by X-ray crystallography.

c) How activity of an enzyme is regulated by irreversible changes in covalent

structure? Explain with example.

d) Study of pre-steady state kinetics determines the mechanism of enzyme

catalysis. Explain.

P.T.O.

Q3) Answer any two of the following :                                                                  [10]

How the rate of degradation (Kd) of the enzyme is measured?

What is positive co-operativity? Explain with suitable example.

Why is chymotrypsin most active at pH 8? Explain its mechanism.

SECTION - II

(Biophysical Techniques)

Q4) Answer any three of the following.                                                                 [15]

a)       Describe DNA cellulose and MAK chromatography.

b)       Write a note on SDS-PAGE.

c)       How does HPTLC give rapid separation and higher resolution?

d)       What do you understand the term ‘finger-printing’? List out its application.

Q5) Answer any three of the following :                                                                 [15]

Draw the schematic diagram of a UV-VIS spectrometer and explain the

instrumentation.

Explain the principle and application of Hydroxyapatite Chromatography.

How can molecules with the same charge at varying amounts be separated

by chromatography?

Explain any two applications of dialysis.

Q6) Answer any two of the following :                                                                   [10]

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