B.E-B.E Biotechnology 1st Sem BIOSEPAREATIONS- I (University of Pune, Pune-2013)

[Total No. of Questions: 12]                        [Total No. of Printed Pages: 4]

UNIVERSITY OF PUNE

[4363]-278

       T. E. (Biotechnology) Examination

M a y - 2013 BIOSEPAREATIONS- I (315468) (2008 Course)

[Time: 3 Hours]                                                                          [Max. Marks: 100]

Instructions:

1 Answers to the two sections should be written in separate

a n s w e r - bo o k s .

2 Black figures to the right indicate full marks.

3 Your answer will be valued as a whole

4 Neat diagrams must be drawn wherever necessary.

5 Assume suitable data, if necessary.

SECTION -I

Q.1               A            What is Ultrasonication ? Discuss various factors         6

affecting Ultrasonication.

B             What is a Bioprocess? Give flow chart of major steps 10

involved in any bioprocess. Explain in detail downstream processing of bioproducts.

OR

Q.2               A            Explain types of biotechnology based on biocatalytic   6

developments.

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B             What are the different types of adsorption isotherms? 6

How these isotherms can be applied?

C             Give importance of centrifugation in bioseparation       4

operations.

Q. 3             A            Explain basis principle of Batch Chromatography? 9

What are the important parameters which affect elution

peaks of column chromatography?

B            Write a short note on the following methods of 9

chromatography:

i)          Frontal Analysis ii)      Elution Analysis

OR

Q. 4             A            What is paper chromatography? Describe the working 6

procedure of paper chromatography with one example

B            What is baseline separation? Explain in detail quality 6

criteria for ideal chromatographic separation

C            A chromatographic separation of a two component 6

samples on a 60 cm column gave the retention of two

solutes X1 and X2 as 2.7 and 3.3 minute with base

widths of the two chromatographic peaks being 0.24

and 0.3 minutes respectively. Calculate :

i)          No of theoretical plates

ii)          Plate Height

iii)        Resolution of two peaks

Q. 5             A            What are the different techniques of centrifugation?      8

Explain in detail gradient centrifugation with

Page 2 of 4

One example

B            A laboratory centrifuge is used to collect yeast cells 8

after fermentation. The centrifuge consists of a number

of cylinders rotated perpendicular to the axis of rotation . During centrifugation the distance between

the surface of liquid and the axis of rotation is R0 = 3

cm, and the distance form the bottom of the cylinder to

that axis is R = 10 cm. The yeast cells can assumed to be spherical, with a diameter of d = 8 x 10-4 cm and a

density of