Jawaharlal Nehru Technological University Hyderabad 2011-2nd Sem M.Tech. Naval Architecture and Ocean Engineering Code No: D0302 M.Techs, ch/il GENETIC ENGINEERING - Question Paper

1. define the construction of cDNA libraries and mention the advantages of
cDNA libraries. [12]
2. elaborate Co-integration and Binary Vectors? discuss. [12]
3. provide an illustrated account of various approaches made for detection of nucleic
acids. [12]
4. How do you identify a PCR product? In a particular PCR amplification
experiment, the product found was shorter than the expected length. provide your
comments on the possible reasons. [12]
5. Write a short note on the following:
a) Chromosome walking.
b) Chromosome jumping. [6+6]
6. Comment briefly on various promoters used for expression in plants. [12]
7. elaborate Transposons? discuss Transposon tagging with examples. [12]
8. elaborate Restriction enzymes? Write about their Nomenclature and about the
various classes of Restriction enzymes. [12]

Earning:   Approval pending.