University of Delhi 2010-1st Year B.Sc PMCS (Physics, Mathematics, Computer Science) Prog 3rd NSTRUMENTAL METHODS OF ANALYSIS UNIVERSITY - Question Paper
[This question paper contains 6 printed pages ]
Your Roll No
5209
B.Sc. Prog./III
AC-302 Instrumental Methods of Analysis
(NC - Admissions of 2005 & onwards)
Maximum Marks 75
3 Hours
Time
(Write your Roll No on the top immediately on receipt of this question paper)
Attempt any six questions in all Question No 1 is compulsory
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(a) Why is radiation source m Atomic Absorption Spectrophotometer usually modulated 9 (3)
(b) Explain, a sample tube is placed after monochromators in UV placed before monochromators in IR spectrojieters (3)
f the following molecules are IR active
(c) Which and wf
9
CH., CO?i HCN and N,0
(3)
(d) Why is it necessary to add NaT at the tune of collection of blood, used for the estimation of blood
(3)
(e) Differentiate between plane polarised radiations
and electromagnetic radiations
(l/2)
(f) Explain, Deshielding due to Hydrogen bonding
(lJ/2)
2 (a) What arc the different electromc-transitions possible in UV region 9 What are their relative energies and which of these is the most intense and why 7 (4)
(b) Define the following (i) Chromophore (n) Auxochrome (in) Hypochromic shift
(iv) Hyperchromic shift
(4)
(c) Find out the probable absorptioposition of the most intense band of these ctnpounds using
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Woodward-Fieser rules COOH | |
 |
Cl |
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Cl | |
(4)
3 (a) What is the necessary criterion for a molecule to giveJR absorption bands 9
How would you distinguish between the following pairs of organic compounds by using IR spectroscopy 7
(1) CH3CH2NH2 and C6H5NH2 (n) CH3CHO and CH3COOH (4)
(b) Sketch the optical path of a double beam IR spectrophotometer and explain why double beam spectrophotometer is preferred over single beam spectrophotometer (4)
(c) Explain, the various types of molecular vibrations associated with IR absorption (2)
(d) How does IR spectroscopy helps in distinguishing between intermolecular and intramolecular hydrogening bonding (2)
4 Write short notes on any three of the following
(I) Relaxation process (as applied in NMR spectroscopy)
I
(II) Total consumption burner and premix burner
(m) Limitations of Lambert-Beers Law
(iv) Saponification value of fats (4,4,4)
(a) What are magnetically active and inactive nuclei ? Explain with suitable examples (3)
(b) A compound having molccular formula C4H802, shows three peaks in PMR spectrum at 5 = 12 (triplet), 6 = 21 (singlet) and 8 = 44 (quartet) respectively Identify the compound (3)
(c) Why is TMS selected as a reference compound in NMR spectroscopy (3)
(d) What are the various factors that influences the magnitude of chemical-shift in NMR spectroscopy7 (3)
(a) Explain in brief, the light source used in atomic Absorption Spectroscopy (4)
(b) Under what circumstances NO - acetylene flame would be preferred over oxyacetylene flame in atomic absorption spectroscopy Explain with examples (4)
(c) Give an account of various interferences that may effect flame emission or flame absorption analysis (4)
7 (a) A reagent X-cleaves selectively those peptide bonds whose carbonyl function is donated by methionene Predict the action of this reagent on the following peptide
G ly-T yr-Ser-A la-Met-G ly-His-Val-H ls-Met-Pro-Phe-Met-Asp (4)
(b) Describe Sanger's method for determination of N-terminal amino acid (3)
(c) Suggest qualitative test to detect the following and give chemical equations wherever possible
(1) Blood glucose
(n) Kctonuria
(m) Bilirubin m blood (5)
% (a) Explain Renal threshold value and explain their
biological significance (3)
(b) Write short Sotes on
Coagulation of blood (3)
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(c) Define specific rotation On what factors does it depend ? (3)
(d) Sketch the optical-path of a polarimeter and precisely describe its functioning (3)
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! (300)****
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