University of Pune 2010-1st Sem M.Sc BioTechnology -Advanced Biological Chemistry Previous -- - Question Paper

M.Sc. Biotechnology - Previous ques. Paper
University of pune (Examination - 2010)

M.Sc. BIOTECHNOLOGY
BT 11 : Advanced Biological Chemistry
(2008 Pattern, New) (Sem. - I)
Time :3Hours] [Max. Marks : 80
Instructions to the candidates:
1) Attempt a total of 5 ques. selecting at lowest 2 ques. from every part.
2) Answers to the 2 parts must be written on separate ans books.
3) Neat diagrams must be drawn wherever necessary.
4) Figures to the right indicate full marks.
P1196

part - I
Q1) a) discuss NMR and IR spectroscopy have been proven to be valuable
techniques in structural biology. [8]

b) With the help of a schematic diagram, discuss the components and
working of spectro fluorometer. [8]

Q2) a) discuss with the help of a suitable example, how pathway manipulation
leads to qualitative and/or quantitative change in the final product. [8]
b) explain the role of HPLC and GLC in analysis of a metabolite. [8]

Q3) a) If the equilibrium concentration of ATP = 1.0 × 10 seven M, ADP = 0.165M
and Pi = 0.1 M, elaborate the equilibrium constants and ? Go for the
hydrolysis of ATP (25°C)? [8]

b) What is meant by metabolic flux analysis? Mention, with suitable example,
its significance. [8]

Q4) a) Illustrate the principle of isopycnic density gradient centrifugation. State
its applications. [8]

b) Enlist the kinds of terpenoids mention 4 major steps involved in the
biosynthesis of terpenoids. [8]

part - II
Q5) a) discuss 2D gel electrophoresis as an improper tool to study proteins. [8]

b) Compare and contrast integral proteins and peripheral proteins with
respect to isolation, properties and functions. [8]

Q6) a) Mention the phytochemical methods for analysis of natural products
and discuss any one. [8]

b) Enlist the kinds of secondary metabolites and mention at lowest on
pharmacological action of every. [8]

Q7) a) define the role of chaperons in assisted protein folding. [8]

b) Write an explanatory note on sequential model of allosterism. [8]

Q8) a) Selecting an improper example, illustrate pathway manipulation at
cellular level. [8]

b) elaborate alkaloids? Mention their kinds. State pharmacological action
of any 4 kinds of alkaloids. [8]

Earning:   Approval pending.