Thapar University 2006 B.Tech Biotechnology Genetic & Metabolic Engineering - Question Paper
Thapar Institute of Engineering & Technology
B.Tech Biotechnology (4th Year)
Final Term exam
BT010 (Genetic & Metabolic Engineering)
THAPAR INSTITUTE OF ENGINEERING AND TECHNOLOGY: PATIALA (Department of Biotechnolog) & Environmental Sciences)
End-Sem Examination (Sem-I, 2006-2007), B.Tech.BiotechnoIogy 4th Year
Genetic & Metabolic Engineering (BT-010)
Maximum Marks: 36 Time: 3.0 Hrs
09-12-2006
Attempt all the questions.
(Answers should be precise and to the point. Provide diagram if required to improve the quality of the answers.)
1. Attempt any three of the following questions: 2.0x3
a) A typical ligation reaction leads to varying products. Justify the statement.
b) Calculate the amount of DNA present in 0.5 ml solution having A2r-0-4.
c) You are given X DNA digested with HindW] enzyme. How do you demonstrate the test ligation using this DNA sample?
d) There is a mixture of two DNA fragments A & B of equal size i.e., 2.5 kb.
The fragment 1B contains an internal HindiW site. How do you purify the fragment A from this mixture?
2. a) Give a diagram of plasmid-based E. coli expression vector. What are the factors
that affect the expression of cloned genes in E. coin . 1.0+2.0
b) Give an example of inducible promoter that drives foreign genes in E. coli.
Give your comments on its usefulness. 2.0
c) Only name any four protein products of recombinant DNA technology. 1.0
OR
a) What are the ingredients required for Sangers method of DNA sequencing? 1.0+2.0 Also mention briefly the underlying principle. What is automated DNA +1.0 sequencing
b) What is the purpose of gel retardation assay? How do you execute the same? 2.0
4. Write a short note along with application of the following (any three) :
a) E.coli DNA Polymerase I
b) BamH\ Methylase
c) T4 Polynucleotide Kinase
d) T7 RNA Polymerase
5. a) Suppose you have a completely sequenced 1275 bp cDNA sequence. Sequence
analysis reveals an ORF from the bases 117 to 746 in one of the strands. Calculate the size and approx. molecular weight of the polypeptide encoded by this predicted ORF.
2.0
2.0
2.0
1.5
1.5
1.5
1.5
b) What are the characteristic features of eukaryotic mRNA. How do you purify this mRNA population?
c) What are the stringency parameters commonly employed during nucleic acid hybridization?
b) How do you check the quality of a gene library? 2.0
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| Earning: Approval pending. |
